Literature DB >> 8692983

Circumventing tolerance to generate autologous monoclonal antibodies to the prion protein.

R A Williamson1, D Peretz, N Smorodinsky, R Bastidas, H Serban, I Mehlhorn, S J DeArmond, S B Prusiner, D R Burton.   

Abstract

Prion diseases are disorders of protein conformation and do not provoke an immune response. Raising antibodies to the prion protein (PrP) has been difficult due to conservation of the PrP sequence and to inhibitory activity of alpha-PrP antibodies toward lymphocytes. To circumvent these problems, we immunized mice in which the PrP gene was ablated (Prnp 0/0) and retrieved specific monoclonal antibodies (mAbs) through phage display libraries. This approach yielded alpha-PrP mAbs that recognize mouse PrP. Studies with these mAbs suggest that cellular PrP adopts an unusually open structure consistent with the conformational plasticity of this protein.

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Year:  1996        PMID： 8692983      PMCID： PMC38974          DOI： 10.1073/pnas.93.14.7279

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  37 in total

1.  Encephalopathy in mice produced by inoculation with scrapie brain material.

Authors:  R L CHANDLER
Journal:  Lancet       Date:  1961-06-24       Impact factor: 79.321

Review 2.  Molten globule and protein folding.

Authors:  O B Ptitsyn
Journal:  Adv Protein Chem       Date:  1995

3.  Conversion of alpha-helices into beta-sheets features in the formation of the scrapie prion proteins.

Authors:  K M Pan; M Baldwin; J Nguyen; M Gasset; A Serban; D Groth; I Mehlhorn; Z Huang; R J Fletterick; F E Cohen
Journal:  Proc Natl Acad Sci U S A       Date:  1993-12-01       Impact factor: 11.205

Review 4.  Human antibodies from combinatorial libraries.

Authors:  D R Burton; C F Barbas
Journal:  Adv Immunol       Date:  1994       Impact factor: 3.543

5.  Conformational transitions, dissociation, and unfolding of scrapie amyloid (prion) protein.

Authors:  J Safar; P P Roller; D C Gajdusek; C J Gibbs
Journal:  J Biol Chem       Date:  1993-09-25       Impact factor: 5.157

6.  Attempts to demonstrate neutralising antibodies in the sera of scrapie-affected animals.

Authors:  M C Clarke; D A Haig
Journal:  Vet Rec       Date:  1966-05-07       Impact factor: 2.695

7.  Human monoclonal antibodies against a plethora of viral pathogens from single combinatorial libraries.

Authors:  R A Williamson; R Burioni; P P Sanna; L J Partridge; C F Barbas; D R Burton
Journal:  Proc Natl Acad Sci U S A       Date:  1993-05-01       Impact factor: 11.205

Review 8.  Biology and genetics of prion diseases.

Authors:  S B Prusiner
Journal:  Annu Rev Microbiol       Date:  1994       Impact factor: 15.500

9.  High-level expression and characterization of a purified 142-residue polypeptide of the prion protein.

Authors:  I Mehlhorn; D Groth; J Stöckel; B Moffat; D Reilly; D Yansura; W S Willett; M Baldwin; R Fletterick; F E Cohen; R Vandlen; D Henner; S B Prusiner
Journal:  Biochemistry       Date:  1996-04-30       Impact factor: 3.162

10.  Neutralizing recombinant human antibodies to a conformational V2- and CD4-binding site-sensitive epitope of HIV-1 gp120 isolated by using an epitope-masking procedure.

Authors:  H J Ditzel; J M Binley; J P Moore; J Sodroski; N Sullivan; L S Sawyer; R M Hendry; W P Yang; C F Barbas; D R Burton
Journal:  J Immunol       Date:  1995-01-15       Impact factor: 5.422
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  25 in total

1.  Immobilized prion protein undergoes spontaneous rearrangement to a conformation having features in common with the infectious form.

Authors:  E Leclerc; D Peretz; H Ball; H Sakurai; G Legname; A Serban; S B Prusiner; D R Burton; R A Williamson
Journal:  EMBO J       Date:  2001-04-02       Impact factor: 11.598

2.  Strain-specified relative conformational stability of the scrapie prion protein.

Authors:  D Peretz; M R Scott; D Groth; R A Williamson; D R Burton; F E Cohen; S B Prusiner
Journal:  Protein Sci       Date:  2001-04       Impact factor: 6.725

3.  Direct evidence of generation and accumulation of β-sheet-rich prion protein in scrapie-infected neuroblastoma cells with human IgG1 antibody specific for β-form prion protein.

Authors:  Toshiya Kubota; Yuta Hamazoe; Shuhei Hashiguchi; Daisuke Ishibashi; Kazuyuki Akasaka; Noriyuki Nishida; Shigeru Katamine; Suehiro Sakaguchi; Ryota Kuroki; Toshihiro Nakashima; Kazuhisa Sugimura
Journal:  J Biol Chem       Date:  2012-02-22       Impact factor: 5.157

4.  Generation of antisera to purified prions in lipid rafts.

Authors:  Robert Hnasko; Ana V Serban; George Carlson; Stanley B Prusiner; Larry H Stanker
Journal:  Prion       Date:  2010-04-09       Impact factor: 3.931

5.  Generation of antibodies against bovine recombinant prion protein in various strains of mice.

Authors:  Olga Andrievskaia; Heather McRae; Cathy Elmgren; Hongsheng Huang; Aru Balachandran; Klaus Nielsen
Journal:  Clin Vaccine Immunol       Date:  2006-01

6.  Phosphorothioate oligonucleotides reduce PrP levels and prion infectivity in cultured cells.

Authors:  Marcela V Karpuj; Kurt Giles; Sagit Gelibter-Niv; Michael R Scott; Vishwanath R Lingappa; Francis C Szoka; David Peretz; Wilfred Denetclaw; Stanley B Prusiner
Journal:  Mol Med       Date:  2007 Mar-Apr       Impact factor: 6.354

7.  Construction of recombinant monoclonal antibodies from a chicken hybridoma line secreting specific antibody.

Authors:  N Nakamura; Y Aoki; H Horiuchi; S Furusawa; H I Yamanaka; T Kitamoto; H Matsuda
Journal:  Cytotechnology       Date:  2000-03       Impact factor: 2.058

8.  Recombinant scrapie-like prion protein of 106 amino acids is soluble.

Authors:  T Muramoto; M Scott; F E Cohen; S B Prusiner
Journal:  Proc Natl Acad Sci U S A       Date:  1996-12-24       Impact factor: 11.205

9.  Processing and degradation of exogenous prion protein by CD11c(+) myeloid dendritic cells in vitro.

Authors:  Katarina M Luhr; Robert P A Wallin; Hans-Gustaf Ljunggren; Peter Löw; Albert Taraboulos; Krister Kristensson
Journal:  J Virol       Date:  2002-12       Impact factor: 5.103

10.  Novel epitopes identified by anti-PrP monoclonal antibodies produced following immunization of Prnp0/0 Balb/cJ mice with purified scrapie prions.

Authors:  Larry H Stanker; Miles C Scotcher; Alice Lin; Jeffery McGarvey; Stanley B Prusiner; Robert Hnasko
Journal:  Hybridoma (Larchmt)       Date:  2012-10
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