Literature DB >> 8686376

pMPY-ZAP: a reusable polymerase chain reaction-directed gene disruption cassette for Saccharomyces cerevisiae.

B L Schneider1, B Steiner, W Seufert, A B Futcher.   

Abstract

Gene disruption is an important method for genetic analysis in Saccharomyces cerevisiae. We have designed a polymerase chain reaction-directed gene disruption cassette that allows rapid disruption of genes in S. cerevisiae without previously cloning them. In addition, this cassette allows recycling of URA3, generating gene disruptions without the permanent loss of the ura3 marker. An indefinite number of disruptions can therefore be made in the same strain.

Entities:  

Mesh：

Substances：

Year:  1996        PMID： 8686376     DOI： 10.1002/(sici)1097-0061(199602)12:2<129::aid-yea891>3.0.co;2-o

Source DB:  PubMed          Journal:  Yeast        ISSN： 0749-503X            Impact factor:   3.239


  23 in total

1.  SCF ubiquitin protein ligases and phosphorylation-dependent proteolysis.

Authors:  A R Willems; T Goh; L Taylor; I Chernushevich; A Shevchenko; M Tyers
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  1999-09-29       Impact factor: 6.237

2.  Coevolution of transcriptional and allosteric regulation at the chorismate metabolic branch point of Saccharomyces cerevisiae.

Authors:  S Krappmann; W N Lipscomb; G H Braus
Journal:  Proc Natl Acad Sci U S A       Date:  2000-12-05       Impact factor: 11.205

3.  Distinct roles for Khd1p in the localization and expression of bud-localized mRNAs in yeast.

Authors:  Yuko Hasegawa; Kenji Irie; André P Gerber
Journal:  RNA       Date:  2008-09-19       Impact factor: 4.942

4.  The 50:50 method for PCR-based seamless genome editing in yeast.

Authors:  Joe Horecka; Ronald W Davis
Journal:  Yeast       Date:  2013-12-13       Impact factor: 3.239

5.  Interactions of Isw2 chromatin remodeling complex with nucleosomal arrays: analyses using recombinant yeast histones and immobilized templates.

Authors:  M E Gelbart; T Rechsteiner; T J Richmond; T Tsukiyama
Journal:  Mol Cell Biol       Date:  2001-03       Impact factor: 4.272

6.  Chromatin remodeling protein Chd1 interacts with transcription elongation factors and localizes to transcribed genes.

Authors:  Rajna Simic; Derek L Lindstrom; Hien G Tran; Kelli L Roinick; Patrick J Costa; Alexander D Johnson; Grant A Hartzog; Karen M Arndt
Journal:  EMBO J       Date:  2003-04-15       Impact factor: 11.598

7.  The Est1 subunit of yeast telomerase binds the Tlc1 telomerase RNA.

Authors:  J Zhou; K Hidaka; B Futcher
Journal:  Mol Cell Biol       Date:  2000-03       Impact factor: 4.272

8.  A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast.

Authors:  U Gueldener; J Heinisch; G J Koehler; D Voss; J H Hegemann
Journal:  Nucleic Acids Res       Date:  2002-03-15       Impact factor: 16.971

9.  When sensing is gambling: An experimental system reveals how plasticity can generate tunable bet-hedging strategies.

Authors:  Colin S Maxwell; Paul M Magwene
Journal:  Evolution       Date:  2017-03-07       Impact factor: 3.694

10.  Spt10-dependent transcriptional activation in Saccharomyces cerevisiae requires both the Spt10 acetyltransferase domain and Spt21.

Authors:  David Hess; Bingsheng Liu; Nadia R Roan; Rolf Sternglanz; Fred Winston
Journal:  Mol Cell Biol       Date:  2004-01       Impact factor: 4.272
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.