Literature DB >> 8682806

Use of PCR to isolate genes encoding sigma54-dependent activators from diverse bacteria.

R I Kaufman1, B T Nixon.   

Abstract

Degenerate PCR probes were used to amplify gene fragments encoding the catalytic domain of sigma54-dependent transcription activators. The procedure should be widely applicable, as it recovered both known and novel gene fragments: 5 from Rhizobium meliloti, 13 from Myxococcus xanthus, and 3 from Bacillus subtilis. No fragments were obtained from Synechococcus sp. strain PCC 7002 or Saccharomyces cerevisiae.

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Year:  1996        PMID： 8682806      PMCID： PMC232662          DOI： 10.1128/jb.178.13.3967-3970.1996

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  12 in total

1.  The phosphorylated form of the enhancer-binding protein NTRC has an ATPase activity that is essential for activation of transcription.

Authors:  D S Weiss; J Batut; K E Klose; J Keener; S Kustu
Journal:  Cell       Date:  1991-10-04       Impact factor: 41.582

2.  Two putative protein-tyrosine kinases identified by application of the polymerase chain reaction.

Authors:  A F Wilks
Journal:  Proc Natl Acad Sci U S A       Date:  1989-03       Impact factor: 11.205

3.  Identification of five putative yeast RNA helicase genes.

Authors:  T H Chang; J Arenas; J Abelson
Journal:  Proc Natl Acad Sci U S A       Date:  1990-02       Impact factor: 11.205

4.  Converting Escherichia coli RNA polymerase into an enhancer-responsive enzyme: role of an NH2-terminal leucine patch in sigma 54.

Authors:  J T Wang; A Syed; M Hsieh; J D Gralla
Journal:  Science       Date:  1995-11-10       Impact factor: 47.728

Review 5.  The sigma 54 bacterial enhancer-binding protein family: mechanism of action and phylogenetic relationship of their functional domains.

Authors:  E Morett; L Segovia
Journal:  J Bacteriol       Date:  1993-10       Impact factor: 3.490

Review 6.  The regulation of nitrogen utilization in enteric bacteria.

Authors:  B Magasanik
Journal:  J Cell Biochem       Date:  1993-01       Impact factor: 4.429

7.  Serine proteases from nematode and protozoan parasites: isolation of sequence homologs using generic molecular probes.

Authors:  J A Sakanari; C E Staunton; A E Eakin; C S Craik; J H McKerrow
Journal:  Proc Natl Acad Sci U S A       Date:  1989-07       Impact factor: 11.205

8.  The central domain of Rhizobium meliloti NifA is sufficient to activate transcription from the R. meliloti nifH promoter.

Authors:  E Huala; F M Ausubel
Journal:  J Bacteriol       Date:  1989-06       Impact factor: 3.490

9.  Conservation between coding and regulatory elements of Rhizobium meliloti and Rhizobium leguminosarum dct genes.

Authors:  J Jiang; B H Gu; L M Albright; B T Nixon
Journal:  J Bacteriol       Date:  1989-10       Impact factor: 3.490

10.  Sequence and domain relationships of ntrC and nifA from Klebsiella pneumoniae: homologies to other regulatory proteins.

Authors:  M Drummond; P Whitty; J Wootton
Journal:  EMBO J       Date:  1986-02       Impact factor: 11.598
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  15 in total

1.  A sigma(54) activator protein necessary for spore differentiation within the fruiting body of Myxococcus xanthus.

Authors:  L Gorski; T Gronewold; D Kaiser
Journal:  J Bacteriol       Date:  2000-05       Impact factor: 3.490

2.  Developmental aggregation of Myxococcus xanthus requires frgA, an frz-related gene.

Authors:  K Cho; A Treuner-Lange; K A O'Connor; D R Zusman
Journal:  J Bacteriol       Date:  2000-12       Impact factor: 3.490

3.  Targeted mutagenesis of sigma54 activator proteins in Myxococcus xanthus.

Authors:  L Gorski; D Kaiser
Journal:  J Bacteriol       Date:  1998-11       Impact factor: 3.490

4.  A protein-induced DNA bend increases the specificity of a prokaryotic enhancer-binding protein.

Authors:  J Dworkin; A J Ninfa; P Model
Journal:  Genes Dev       Date:  1998-03-15       Impact factor: 11.361

5.  A cascade of coregulating enhancer binding proteins initiates and propagates a multicellular developmental program.

Authors:  Krista M Giglio; Nora Caberoy; Garret Suen; Dale Kaiser; Anthony G Garza
Journal:  Proc Natl Acad Sci U S A       Date:  2011-06-13       Impact factor: 11.205

6.  Identification of a positive transcription regulatory element within the coding region of the nifLA operon in Azotobacter vinelandii.

Authors:  Ranjana Mitra; Hirendra K Das; Aparna Dixit
Journal:  Appl Environ Microbiol       Date:  2005-07       Impact factor: 4.792

7.  Role of bkdR, a transcriptional activator of the sigL-dependent isoleucine and valine degradation pathway in Bacillus subtilis.

Authors:  M Debarbouille; R Gardan; M Arnaud; G Rapoport
Journal:  J Bacteriol       Date:  1999-04       Impact factor: 3.490

8.  Genetic studies of mrp, a locus essential for cellular aggregation and sporulation of Myxococcus xanthus.

Authors:  H Sun; W Shi
Journal:  J Bacteriol       Date:  2001-08       Impact factor: 3.490

9.  Functional characterization of Vibrio alginolyticus twin-arginine translocation system: its roles in biofilm formation, extracellular protease activity, and virulence towards fish.

Authors:  Honghong He; Qiyao Wang; Lili Sheng; Qin Liu; Yuanxing Zhang
Journal:  Curr Microbiol       Date:  2010-12-18       Impact factor: 2.188

10.  sigma54, a vital protein for Myxococcus xanthus.

Authors:  I M Keseler; D Kaiser
Journal:  Proc Natl Acad Sci U S A       Date:  1997-03-04       Impact factor: 11.205
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