Literature DB >> 8680932

A general assay for restriction endonucleases and other DNA-modifying enzymes with plasmid substrates.

I B Vipond1, G S Baldwin, M Oram, S G Erskine, L M Wentzell, M D Szczelkun, T J Nobbs, S E Halford.   

Abstract

A procedure for measuring the activities of enzymes that alter the covalent structure of DNA is described. The assay utilizes covalently closed circles of DNA as the substrate and yields quantitative data on the fraction of this DNA converted to both open-circle and linear forms.

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Year:  1995        PMID： 8680932     DOI： 10.1007/BF02779019

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  26 in total

Review 1.  Facilitated target location in biological systems.

Authors:  P H von Hippel; O G Berg
Journal:  J Biol Chem       Date:  1989-01-15       Impact factor: 5.157

2.  The problems of eukaryotic and prokaryotic DNA packaging and in vivo conformation posed by superhelix density heterogeneity.

Authors:  M Shure; D E Pulleyblank; J Vinograd
Journal:  Nucleic Acids Res       Date:  1977       Impact factor: 16.971

Review 3.  Mechanism of specific site location and DNA cleavage by EcoR I endonuclease.

Authors:  B J Terry; W E Jack; P Modrich
Journal:  Gene Amplif Anal       Date:  1987

4.  EcoRI methylase. Physical and catalytic properties of the homogeneous enzyme.

Authors:  R A Rubin; P Modrich
Journal:  J Biol Chem       Date:  1977-10-25       Impact factor: 5.157

5.  The interaction of closed circular DNA with intercalative dyes. I. The superhelix density of SV40 DNA in the presence and absence of dye.

Authors:  W Bauer; J Vinograd
Journal:  J Mol Biol       Date:  1968-04-14       Impact factor: 5.469

6.  The cloning, purification and characterization of the Eco RV modification methylase.

Authors:  V U Nwosu; B A Connolly; S E Halford; J Garnett
Journal:  Nucleic Acids Res       Date:  1988-05-11       Impact factor: 16.971

7.  In vitro analysis of Hin-mediated site-specific recombination.

Authors:  R C Johnson; M B Bruist; M B Glaccum; M I Simon
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1984

8.  Site-specific recombination and topoisomerization by Tn21 resolvase: role of metal ions.

Authors:  S E Castell; S L Jordan; S E Halford
Journal:  Nucleic Acids Res       Date:  1986-09-25       Impact factor: 16.971

9.  The effect of divalent cations on the mode of action of DNase I. The initial reaction products produced from covalently closed circular DNA.

Authors:  V W Campbell; D A Jackson
Journal:  J Biol Chem       Date:  1980-04-25       Impact factor: 5.157

10.  Preferential site-specific hemimethylation of GATC sites in pBR322 DNA by Dam methyltransferase from Escherichia coli.

Authors:  A Bergerat; A Kriebardis; W Guschlbauer
Journal:  J Biol Chem       Date:  1989-03-05       Impact factor: 5.157
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  13 in total

1.  DNA communications by Type III restriction endonucleases--confirmation of 1D translocation over 3D looping.

Authors:  Luke J Peakman; Mark D Szczelkun
Journal:  Nucleic Acids Res       Date:  2004-08-09       Impact factor: 16.971

2.  Repercussions of DNA tracking by the type IC restriction endonuclease EcoR124I on linear, circular and catenated substrates.

Authors:  M D Szczelkun; M S Dillingham; P Janscak; K Firman; S E Halford
Journal:  EMBO J       Date:  1996-11-15       Impact factor: 11.598

3.  Recycling of protein subunits during DNA translocation and cleavage by Type I restriction-modification enzymes.

Authors:  Michelle Simons; Mark D Szczelkun
Journal:  Nucleic Acids Res       Date:  2011-06-28       Impact factor: 16.971

4.  DNA cleavage site selection by Type III restriction enzymes provides evidence for head-on protein collisions following 1D bidirectional motion.

Authors:  Friedrich W Schwarz; Kara van Aelst; Júlia Tóth; Ralf Seidel; Mark D Szczelkun
Journal:  Nucleic Acids Res       Date:  2011-06-30       Impact factor: 16.971

5.  Concerted action at eight phosphodiester bonds by the BcgI restriction endonuclease.

Authors:  Jacqueline J T Marshall; Rachel M Smith; Sumita Ganguly; Stephen E Halford
Journal:  Nucleic Acids Res       Date:  2011-06-07       Impact factor: 16.971

6.  Characterization of the Type III restriction endonuclease PstII from Providencia stuartii.

Authors:  Alice Sears; Luke J Peakman; Geoffrey G Wilson; Mark D Szczelkun
Journal:  Nucleic Acids Res       Date:  2005-08-24       Impact factor: 16.971

7.  DNA cleavage and methylation specificity of the single polypeptide restriction-modification enzyme LlaGI.

Authors:  Rachel M Smith; Fiona M Diffin; Nigel J Savery; Jytte Josephsen; Mark D Szczelkun
Journal:  Nucleic Acids Res       Date:  2009-11       Impact factor: 16.971

8.  The Type ISP Restriction-Modification enzymes LlaBIII and LlaGI use a translocation-collision mechanism to cleave non-specific DNA distant from their recognition sites.

Authors:  Eva Šišáková; Kara van Aelst; Fiona M Diffin; Mark D Szczelkun
Journal:  Nucleic Acids Res       Date:  2012-12-07       Impact factor: 16.971

9.  DNA cleavage by Type ISP Restriction-Modification enzymes is initially targeted to the 3'-5' strand.

Authors:  Kara van Aelst; Eva Šišáková; Mark D Szczelkun
Journal:  Nucleic Acids Res       Date:  2012-12-05       Impact factor: 16.971

10.  Motor step size and ATP coupling efficiency of the dsDNA translocase EcoR124I.

Authors:  Ralf Seidel; Joost G P Bloom; Cees Dekker; Mark D Szczelkun
Journal:  EMBO J       Date:  2008-04-03       Impact factor: 11.598
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