Literature DB >> 8680931

One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin.

J Crowe1, B S Masone, J Ribbe.   

Abstract

The 6xHis/Ni-NTA system allows rapid and efficient affinity purification of recombinant proteins from virtually any expression system. Protocols and tips for purification under both native and denaturing conditions are provided, as well as a rapid spin procedure for protein minipreps.

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Year:  1995        PMID： 8680931     DOI： 10.1007/BF02779018

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  17 in total

1.  Metal chelate affinity chromatography, a new approach to protein fractionation.

Authors:  J Porath; J Carlsson; I Olsson; G Belfrage
Journal:  Nature       Date:  1975-12-18       Impact factor: 49.962

2.  A novel sequential procedure to enhance the renaturation of recombinant protein from Escherichia coli inclusion bodies.

Authors:  B Fischer; B Perry; I Sumner; P Goodenough
Journal:  Protein Eng       Date:  1992-09

3.  Physical interaction between heat shock proteins DnaK, DnaJ, and GrpE and the bacterial heat shock transcription factor sigma 32.

Authors:  J Gamer; H Bujard; B Bukau
Journal:  Cell       Date:  1992-05-29       Impact factor: 41.582

4.  Preparation of clinical grade proteins produced by recombinant DNA technologies.

Authors:  B J Takacs; M F Girard
Journal:  J Immunol Methods       Date:  1991-10-25       Impact factor: 2.303

5.  Expression and purification of the leucine zipper and DNA-binding domains of Fos and Jun: both Fos and Jun contact DNA directly.

Authors:  C Abate; D Luk; R Gentz; F J Rauscher; T Curran
Journal:  Proc Natl Acad Sci U S A       Date:  1990-02       Impact factor: 11.205

6.  Rapid purification of homodimer and heterodimer HIV-1 reverse transcriptase by metal chelate affinity chromatography.

Authors:  S F Le Grice; F Grüninger-Leitch
Journal:  Eur J Biochem       Date:  1990-01-26

7.  Ligand binding assays with recombinant proteins refolded on an affinity matrix.

Authors:  D Sinha; M Bakhshi; R Vora
Journal:  Biotechniques       Date:  1994-09       Impact factor: 1.993

8.  Production, purification and immunogenicity of a malaria transmission-blocking vaccine candidate: TBV25H expressed in yeast and purified using nickel-NTA agarose.

Authors:  D C Kaslow; J Shiloach
Journal:  Biotechnology (N Y)       Date:  1994-05

9.  Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus.

Authors:  R Janknecht; G de Martynoff; J Lou; R A Hipskind; A Nordheim; H G Stunnenberg
Journal:  Proc Natl Acad Sci U S A       Date:  1991-10-15       Impact factor: 11.205

10.  Major surface antigen p190 of Plasmodium falciparum: detection of common epitopes present in a variety of plasmodia isolates.

Authors:  R Gentz; U Certa; B Takacs; H Matile; H Döbeli; R Pink; M Mackay; N Bone; J G Scaife
Journal:  EMBO J       Date:  1988-01       Impact factor: 11.598
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  14 in total

1.  Synthesis of poly(N-isopropylacrylamide) particles for metal affinity binding of peptides.

Authors:  Hsin-Yi Tsai; Alexander Lee; Wei Peng; Matthew Z Yates
Journal:  Colloids Surf B Biointerfaces       Date:  2013-10-11       Impact factor: 5.268

2.  Mutation of a single MalK subunit severely impairs maltose transport activity in Escherichia coli.

Authors:  A L Davidson; S Sharma
Journal:  J Bacteriol       Date:  1997-09       Impact factor: 3.490

3.  The Power of Human Protective Modifiers: PLS3 and CORO1C Unravel Impaired Endocytosis in Spinal Muscular Atrophy and Rescue SMA Phenotype.

Authors:  Seyyedmohsen Hosseinibarkooie; Miriam Peters; Laura Torres-Benito; Raphael H Rastetter; Kristina Hupperich; Andrea Hoffmann; Natalia Mendoza-Ferreira; Anna Kaczmarek; Eva Janzen; Janine Milbradt; Tobias Lamkemeyer; Frank Rigo; C Frank Bennett; Christoph Guschlbauer; Ansgar Büschges; Matthias Hammerschmidt; Markus Riessland; Min Jeong Kye; Christoph S Clemen; Brunhilde Wirth
Journal:  Am J Hum Genet       Date:  2016-08-04       Impact factor: 11.025

4.  An examination of the binding behavior of histidine-containing peptides with immobilized metal complexes derived from the macrocyclic ligand, 1,4,7-triazacyclononane.

Authors:  Bim Graham; Peter Comba; Milton T W Hearn; Leone Spiccia
Journal:  J Biol Inorg Chem       Date:  2006-09-09       Impact factor: 3.358

5.  Purification of the pyruvate dehydrogenase multienzyme complex of Zymomonas mobilis and identification and sequence analysis of the corresponding genes.

Authors:  U Neveling; R Klasen; S Bringer-Meyer; H Sahm
Journal:  J Bacteriol       Date:  1998-03       Impact factor: 3.490

6.  Identification and Characterization of the Two-Component System HK8700-RR8701 of Kocuria rhizophila DC2201.

Authors:  Baohua Chen; Tingting Zou; Long Zou; Haiyan Ni; Yunhong Huang; Zhong-Er Long
Journal:  Protein J       Date:  2019-12       Impact factor: 2.371

7.  ß3 integrin modulates transforming growth factor beta induced (TGFBI) function and paclitaxel response in ovarian cancer cells.

Authors:  David A Tumbarello; Jillian Temple; James D Brenton
Journal:  Mol Cancer       Date:  2012-05-28       Impact factor: 27.401

8.  Hexahistidine-tag-specific optical probes for analyses of proteins and their interactions.

Authors:  Chunxia Zhao; Lance M Hellman; Xin Zhan; Willis S Bowman; Sidney W Whiteheart; Michael G Fried
Journal:  Anal Biochem       Date:  2009-12-28       Impact factor: 3.365

9.  Efficient generation of rat induced pluripotent stem cells using a non-viral inducible vector.

Authors:  Claudia Merkl; Anja Saalfrank; Nathalie Riesen; Ralf Kühn; Anna Pertek; Stefan Eser; Markus Sebastian Hardt; Alexander Kind; Dieter Saur; Wolfgang Wurst; Antonio Iglesias; Angelika Schnieke
Journal:  PLoS One       Date:  2013-01-31       Impact factor: 3.240

10.  Induction of innate immune signatures following polyepitope protein-glycoprotein B-TLR4&9 agonist immunization generates multifunctional CMV-specific cellular and humoral immunity.

Authors:  Vijayendra Dasari; Corey Smith; Andrea Schuessler; Jie Zhong; Rajiv Khanna
Journal:  Hum Vaccin Immunother       Date:  2014-01-24       Impact factor: 3.452
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