UNLABELLED: The fastest and most reliable preparation of radiolabeled 5-iodo-2'- deoxyuridine (*IUdR) is accomplished by iododemetallation. METHODS: We describe a kit formulation for the preparation of *IUdR by demercuration whereby [123I/125I/131I]IUdR is synthesized virtually instantaneously following the incubation of an aqueous solution of the chloromercuric precursor with Na123I/125I/131I in the presence of lodogen. We also report the conditions for the radiosynthesis of IUdR by destannylation of the tributylstannyl precursor using hydrogen peroxide as the oxidant. RESULTS: In each case, the total procedure is completed in 5 min. HPLC indicates the total transformation of iodide into IUdR with no detectable UV-absorbing by-products. The metal content of the sample is low. The product, therefore, does not require purification. CONCLUSION: *IUdR can be prepared instantly, by either demercuration of ClHgUdR or destannylation of Bu3SnUdR. The use of a mercuric precursor favors a kit formulation since the metallic derivative is stable when kept in aqueous solution, aliquoted in vials coated with the oxidant, for up to 3 mo.
UNLABELLED: The fastest and most reliable preparation of radiolabeled 5-iodo-2'- deoxyuridine (*IUdR) is accomplished by iododemetallation. METHODS: We describe a kit formulation for the preparation of *IUdR by demercuration whereby [123I/125I/131I]IUdR is synthesized virtually instantaneously following the incubation of an aqueous solution of the chloromercuric precursor with Na123I/125I/131I in the presence of lodogen. We also report the conditions for the radiosynthesis of IUdR by destannylation of the tributylstannyl precursor using hydrogen peroxide as the oxidant. RESULTS: In each case, the total procedure is completed in 5 min. HPLC indicates the total transformation of iodide into IUdR with no detectable UV-absorbing by-products. The metal content of the sample is low. The product, therefore, does not require purification. CONCLUSION: *IUdR can be prepared instantly, by either demercuration of ClHgUdR or destannylation of Bu3SnUdR. The use of a mercuric precursor favors a kit formulation since the metallic derivative is stable when kept in aqueous solution, aliquoted in vials coated with the oxidant, for up to 3 mo.
Authors: Florence Perillo-Adamer; Angelika Bischof Delaloye; Céline S Genton; Andreas O Schaffland; Yves M Dupertuis; Franz Buchegger Journal: Eur J Nucl Med Mol Imaging Date: 2006-02-01 Impact factor: 9.236
Authors: David Viertl; Angelika Bischof Delaloye; Bernard Lanz; Carole Poitry-Yamate; Rolf Gruetter; Vladimir Mlynarik; Simon M Ametamey; Tobias L Ross; Hans-Anton Lehr; Pierre-Alain André; Florence Perillo-Adamer; Marek Kosinski; Yves M Dupertuis; Franz Buchegger Journal: Mol Imaging Biol Date: 2011-04 Impact factor: 3.488