| Literature DB >> 8675023 |
Abstract
Four new plasmids containing the ermC' (encoding a methyltransferase which confers resistance to erythromycin), xylE-ermC' (xylE, encoding catechol 2,3-dioxygenase) and lacZ-ermC' cassettes have been constructed. The 10-bp gonococcal uptake sequence has been placed downstream from ermC' to facilitate the delivery of these cassettes into pathogenic Neisseria spp. Several restriction sites have been placed to flank the cassettes to allow their excision and directional cloning. These plasmids will provide valuable tools for constructing insertional mutants and transcriptional fusions in Neisseria spp. or other bacteria.Entities:
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Year: 1996 PMID: 8675023 DOI: 10.1016/0378-1119(96)00103-5
Source DB: PubMed Journal: Gene ISSN: 0378-1119 Impact factor: 3.688