Literature DB >> 8675023

Plasmids with erythromycin resistance and catechol 2,3-dioxygenase- or beta-galactosidase-encoding gene cassettes for use in Neisseria spp.

D Zhou1, M A Apicella.   

Abstract

Four new plasmids containing the ermC' (encoding a methyltransferase which confers resistance to erythromycin), xylE-ermC' (xylE, encoding catechol 2,3-dioxygenase) and lacZ-ermC' cassettes have been constructed. The 10-bp gonococcal uptake sequence has been placed downstream from ermC' to facilitate the delivery of these cassettes into pathogenic Neisseria spp. Several restriction sites have been placed to flank the cassettes to allow their excision and directional cloning. These plasmids will provide valuable tools for constructing insertional mutants and transcriptional fusions in Neisseria spp. or other bacteria.

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Year:  1996        PMID: 8675023     DOI: 10.1016/0378-1119(96)00103-5

Source DB:  PubMed          Journal:  Gene        ISSN: 0378-1119            Impact factor:   3.688


  9 in total

1.  Gonococcal genes encoding transferrin-binding proteins A and B are arranged in a bicistronic operon but are subject to differential expression.

Authors:  C Ronpirin; A E Jerse; C N Cornelissen
Journal:  Infect Immun       Date:  2001-10       Impact factor: 3.441

2.  Insertion-duplication mutagenesis of neisseria: use in characterization of DNA transfer genes in the gonococcal genetic island.

Authors:  H L Hamilton; K J Schwartz; J P Dillard
Journal:  J Bacteriol       Date:  2001-08       Impact factor: 3.490

3.  Transcriptional regulation of divergent capsule biosynthesis and transport operon promoters in serogroup B Neisseria meningitidis.

Authors:  Y L Tzeng; J S Swartley; Y K Miller; R E Nisbet; L J Liu; J H Ahn; D S Stephens
Journal:  Infect Immun       Date:  2001-04       Impact factor: 3.441

4.  Endotoxin of Neisseria meningitidis composed only of intact lipid A: inactivation of the meningococcal 3-deoxy-D-manno-octulosonic acid transferase.

Authors:  Yih-Ling Tzeng; Anup Datta; V Kumar Kolli; Russell W Carlson; David S Stephens
Journal:  J Bacteriol       Date:  2002-05       Impact factor: 3.490

5.  Regulatory role of the MisR/S two-component system in hemoglobin utilization in Neisseria meningitidis.

Authors:  Shuming Zhao; Grisselle E Montanez; Pradeep Kumar; Soma Sannigrahi; Yih-Ling Tzeng
Journal:  Infect Immun       Date:  2009-12-14       Impact factor: 3.441

6.  Identification of a novel anti-sigmaE factor in Neisseria meningitidis.

Authors:  Carla Th P Hopman; Dave Speijer; Arie van der Ende; Yvonne Pannekoek
Journal:  BMC Microbiol       Date:  2010-06-04       Impact factor: 3.605

7.  Erythromycin-resistant Neisseria gonorrhoeae and oral commensal Neisseria spp. carry known rRNA methylase genes.

Authors:  M C Roberts; W O Chung; D Roe; M Xia; C Marquez; G Borthagaray; W L Whittington; K K Holmes
Journal:  Antimicrob Agents Chemother       Date:  1999-06       Impact factor: 5.191

8.  Molecular characterization and identification of proteins regulated by Hfq in Neisseria meningitidis.

Authors:  Yvonne Pannekoek; Robert Huis in 't Veld; Carla Th P Hopman; Ankie A J Langerak; Dave Speijer; Arie van der Ende
Journal:  FEMS Microbiol Lett       Date:  2009-04-03       Impact factor: 2.742

9.  Attachment and invasion of Neisseria meningitidis to host cells is related to surface hydrophobicity, bacterial cell size and capsule.

Authors:  Stephanie N Bartley; Yih-Ling Tzeng; Kathryn Heel; Chiang W Lee; Shakeel Mowlaboccus; Torsten Seemann; Wei Lu; Ya-Hsun Lin; Catherine S Ryan; Christopher Peacock; David S Stephens; John K Davies; Charlene M Kahler
Journal:  PLoS One       Date:  2013-02-06       Impact factor: 3.240

  9 in total

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