PURPOSE: The purpose of this study was to develop a new method to produce albumin particles in the sub-200-nanometer range with a narrow size distribution and in a controlled and reproducible manner. METHODS: A new emulsion crosslinking method was developed using ultrasound and static mixing as homogenization steps and a central composite design was used to evaluate the influence of different process parameters on particle size, polydispersity and yield. RESULTS: Response surface analysis allowed the location of the most important factors. Of all the factors investigated, only the albumin concentration and the aqueous phase volume showed a significant influence on response parameters. Albumin nanospheres with sizes below 200 nm in diameter and very narrow size distributions were obtained in high yields ( > 80%). CONCLUSIONS: This study describes a new preparation method for albumin nanoparticles which are suitable for future drug targeting studies.
PURPOSE: The purpose of this study was to develop a new method to produce albumin particles in the sub-200-nanometer range with a narrow size distribution and in a controlled and reproducible manner. METHODS: A new emulsion crosslinking method was developed using ultrasound and static mixing as homogenization steps and a central composite design was used to evaluate the influence of different process parameters on particle size, polydispersity and yield. RESULTS: Response surface analysis allowed the location of the most important factors. Of all the factors investigated, only the albumin concentration and the aqueous phase volume showed a significant influence on response parameters. Albumin nanospheres with sizes below 200 nm in diameter and very narrow size distributions were obtained in high yields ( > 80%). CONCLUSIONS: This study describes a new preparation method for albumin nanoparticles which are suitable for future drug targeting studies.
Authors: M M Stollenwerk; I Pashkunova-Martic; C Kremser; H Talasz; G C Thurner; A A Abdelmoez; E A Wallnöfer; A Helbok; E Neuhauser; N Klammsteiner; L Klimaschewski; E von Guggenberg; E Fröhlich; B Keppler; W Jaschke; P Debbage Journal: Histochem Cell Biol Date: 2010-02-20 Impact factor: 4.304
Authors: Guilin Wang; Kevin Siggers; Sufeng Zhang; Hongxing Jiang; Zhenghe Xu; Ronald F Zernicke; John Matyas; Hasan Uludağ Journal: Pharm Res Date: 2008-08-15 Impact factor: 4.200