Literature DB >> 8660608

A combined high-performance liquid chromatographic-microbiological assay for serum folic acid.

P Kelly1, J McPartlin, J Scott.   

Abstract

An assay of folic acid in human serum is described involving HPLC fractionation of deproteinized serum prior to Lactobacillus casei assay. High specific activity [3H]folic acid is added as internal standard to 1 ml serum prior to deproteinization with perchlorate and an aliquot is applied to a C18 reverse-phase HPLC column. The folic acid fraction was assayed by L. casei microtiter plate assay. Intra- and interassay variations (CV) were 5.5 and 7.5% respectively for sera (n = 10) spiked with folic acid. The percentage recovery for folic acid additions of 10 and 20 ng/liter were 105.2 and 103.7%, respectively. The lower limit of detection was 1 ng folic acid/ml serum. Folates other than folic acid and 5-methyltetrahydrofolate were not detected. The assay permitted serial measurements to be made of folates in serum following an oral dose.

Entities:  

Mesh：

Substances：

Year:  1996        PMID： 8660608     DOI： 10.1006/abio.1996.0272

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  11 in total

1.  Antiatherogenic and radioprotective role of folic acid in whole body gamma-irradiated mice.

Authors:  Madan Sadashiv Pote; Nitin Motilal Gandhi; Kaushala Prasad Mishra
Journal:  Mol Cell Biochem       Date:  2006-09-23       Impact factor: 3.396

2.  Knowledge gaps in understanding the metabolic and clinical effects of excess folates/folic acid: a summary, and perspectives, from an NIH workshop.

Authors:  Padma Maruvada; Patrick J Stover; Joel B Mason; Regan L Bailey; Cindy D Davis; Martha S Field; Richard H Finnell; Cutberto Garza; Ralph Green; Jean-Louis Gueant; Paul F Jacques; David M Klurfeld; Yvonne Lamers; Amanda J MacFarlane; Joshua W Miller; Anne M Molloy; Deborah L O'Connor; Christine M Pfeiffer; Nancy A Potischman; Joseph V Rodricks; Irwin H Rosenberg; Sharon A Ross; Barry Shane; Jacob Selhub; Sally P Stabler; Jacquetta Trasler; Sedigheh Yamini; Giovanna Zappalà
Journal:  Am J Clin Nutr       Date:  2020-11-11       Impact factor: 7.045

Review 3.  Biomarkers of Nutrition for Development-Folate Review.

Authors:  Lynn B Bailey; Patrick J Stover; Helene McNulty; Michael F Fenech; Jesse F Gregory; James L Mills; Christine M Pfeiffer; Zia Fazili; Mindy Zhang; Per M Ueland; Anne M Molloy; Marie A Caudill; Barry Shane; Robert J Berry; Regan L Bailey; Dorothy B Hausman; Ramkripa Raghavan; Daniel J Raiten
Journal:  J Nutr       Date:  2015-06-03       Impact factor: 4.798

Review 4.  Folate, cancer risk, and the Greek god, Proteus: a tale of two chameleons.

Authors:  Joel B Mason
Journal:  Nutr Rev       Date:  2009-04       Impact factor: 7.110

5.  Circulating unmetabolized folic Acid: relationship to folate status and effect of supplementation.

Authors:  Carolyn Tam; Deborah O'Connor; Gideon Koren
Journal:  Obstet Gynecol Int       Date:  2012-02-19

6.  Folic acid fortification and public health: report on threshold doses above which unmetabolised folic acid appear in serum.

Authors:  Mary Rose Sweeney; Joseph McPartlin; John Scott
Journal:  BMC Public Health       Date:  2007-03-22       Impact factor: 3.295

7.  Folic acid supplementation in children with sickle cell disease: study protocol for a double-blind randomized cross-over trial.

Authors:  Brock A Williams; Heather McCartney; Erin Adams; Angela M Devlin; Joel Singer; Suzanne Vercauteren; John K Wu; Crystal D Karakochuk
Journal:  Trials       Date:  2020-06-29       Impact factor: 2.279

8.  Persistent circulating unmetabolised folic acid in a setting of liberal voluntary folic acid fortification. Implications for further mandatory fortification?

Authors:  Mary R Sweeney; Anthony Staines; Leslie Daly; Aisling Traynor; Sean Daly; Steven W Bailey; Patricia B Alverson; June E Ayling; John M Scott
Journal:  BMC Public Health       Date:  2009-08-18       Impact factor: 3.295

9.  Folic Acid Impairs the Uptake of 5-Methyltetrahydrofolate in Human Umbilical Vascular Endothelial Cells.

Authors:  Desiree Smith; Jacqueline Hornstra; Monica Rocha; Gerrit Jansen; Yehuda Assaraf; Inbal Lasry; Henk Blom; Yvo M Smulders
Journal:  J Cardiovasc Pharmacol       Date:  2017-10       Impact factor: 3.105

10.  Is natural (6S)-5-methyltetrahydrofolic acid as effective as synthetic folic acid in increasing serum and red blood cell folate concentrations during pregnancy? A proof-of-concept pilot study.

Authors:  Kelsey M Cochrane; Chantal Mayer; Angela M Devlin; Rajavel Elango; Jennifer A Hutcheon; Crystal D Karakochuk
Journal:  Trials       Date:  2020-05-05       Impact factor: 2.279
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.