Literature DB >> 8660588

Coupled enzymatic assay for estimation of branched-chain L-amino acid aminotransferase activity with 2-Oxo acid substrates.

P Schadewaldt1, F Adelmeyer.   

Abstract

A convenient continuous spectrophotometric assay for estimation of branched-chain L-amino acid aminotransferase activity was established: Branched-chain 2-oxo acid-dependent transamination of L-glutamate was coupled-via 2-oxoglutarate-to L-aspartate aminotransferase plus L-malate dehydrogenase or to L-alanine aminotransferase plus L-lactate dehydrogenase as indicator systems. The rate of transamination can be monitored specifically by measuring the decrease in NADH absorbance at 334 nm over time. The method was applied, e.g., for evaluation of some kinetic properties of the rat heart (iso)enzyme.

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Year:  1996        PMID： 8660588     DOI： 10.1006/abio.1996.0252

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  8 in total

1.  Branched-chain aminotransferase4 is part of the chain elongation pathway in the biosynthesis of methionine-derived glucosinolates in Arabidopsis.

Authors:  Joachim Schuster; Tanja Knill; Michael Reichelt; Jonathan Gershenzon; Stefan Binder
Journal:  Plant Cell       Date:  2006-10-20       Impact factor: 11.277

2.  The feasibility of assessing branched-chain amino acid metabolism in cellular models of prostate cancer with hyperpolarized [1-(13)C]-ketoisocaproate.

Authors:  Kelvin L Billingsley; Jae Mo Park; Sonal Josan; Ralph Hurd; Dirk Mayer; Eleanor Spielman-Sun; Dwight G Nishimura; James D Brooks; Daniel Spielman
Journal:  Magn Reson Imaging       Date:  2014-04-28       Impact factor: 2.546

3.  The mitochondrial branched-chain aminotransferase (AtBCAT-1) is capable to initiate degradation of leucine, isoleucine and valine in almost all tissues in Arabidopsis thaliana.

Authors:  Joachim Schuster; Stefan Binder
Journal:  Plant Mol Biol       Date:  2005-01       Impact factor: 4.076

4.  Arabidopsis branched-chain aminotransferase 3 functions in both amino acid and glucosinolate biosynthesis.

Authors:  Tanja Knill; Joachim Schuster; Michael Reichelt; Jonathan Gershenzon; Stefan Binder
Journal:  Plant Physiol       Date:  2007-12-27       Impact factor: 8.340

5.  Crystal structures of complexes of the branched-chain aminotransferase from Deinococcus radiodurans with α-ketoisocaproate and L-glutamate suggest the radiation resistance of this enzyme for catalysis.

Authors:  Chung-De Chen; Chih-Hao Lin; Phimonphan Chuankhayan; Yen-Chieh Huang; Yin-Cheng Hsieh; Tien-Feng Huang; Hong-Hsiang Guan; Ming-Yih Liu; Wen-Chang Chang; Chun-Jung Chen
Journal:  J Bacteriol       Date:  2012-09-14       Impact factor: 3.490

6.  Arabidopsis and maize RidA proteins preempt reactive enamine/imine damage to branched-chain amino acid biosynthesis in plastids.

Authors:  Thomas D Niehaus; Thuy N D Nguyen; Satinder K Gidda; Mona ElBadawi-Sidhu; Jennifer A Lambrecht; Donald R McCarty; Diana M Downs; Arthur J L Cooper; Oliver Fiehn; Robert T Mullen; Andrew D Hanson
Journal:  Plant Cell       Date:  2014-07-28       Impact factor: 11.277

7.  In vivo assessment of increased oxidation of branched-chain amino acids in glioblastoma.

Authors:  Eul Hyun Suh; Edward P Hackett; R Max Wynn; David T Chuang; Bo Zhang; Weibo Luo; A Dean Sherry; Jae Mo Park
Journal:  Sci Rep       Date:  2019-01-23       Impact factor: 4.379

8.  Serum Removal from Culture Induces Growth Arrest, Ploidy Alteration, Decrease in Infectivity and Differential Expression of Crucial Genes in Leishmania infantum Promastigotes.

Authors:  Pedro J Alcolea; Ana Alonso; Miguel A Moreno-Izquierdo; María A Degayón; Inmaculada Moreno; Vicente Larraga
Journal:  PLoS One       Date:  2016-03-09       Impact factor: 3.240
  8 in total

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