Literature DB >> 8660584

Spectrophotometric assay for processes involving changes in hydrogen ion concentration in aqueous solution.

P G Penketh1, K Shyam, C L Patton, A C Sartorelli.   

Abstract

A strategy for the design of simple colorimetric assays to follow any process that involves the net generation or consumption of hydrogen ions in aqueous solutions over the pH range of 3-10 is described. This procedure relies upon the measurement of the change in absorption when a weakly or moderately buffered solution of a pH indicator is subjected to a small change in pH. Buffers and indicators are chosen with closely matching pKa values. The versatility of this type of assay technique is illustrated using three examples.

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Year:  1996        PMID： 8660584     DOI： 10.1006/abio.1996.0248

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  4 in total

1.  Chloroethylating and methylating dual function antineoplastic agents display superior cytotoxicity against repair proficient tumor cells.

Authors:  Rui Zhu; Raymond P Baumann; Eric Patridge; Philip G Penketh; Krishnamurthy Shyam; Kimiko Ishiguro; Alan C Sartorelli
Journal:  Bioorg Med Chem Lett       Date:  2013-01-11       Impact factor: 2.823

2.  Influence of phosphate and phosphoesters on the decomposition pathway of 1,2-bis(methylsulfonyl)-1-(2-chloroethyhydrazine (90CE), the active anticancer moiety generated by Laromustine, KS119, and KS119W.

Authors:  Philip G Penketh; Krishnamurthy Shyam; Rui Zhu; Raymond P Baumann; Kimiko Ishiguro; Alan C Sartorelli
Journal:  Chem Res Toxicol       Date:  2014-03-24       Impact factor: 3.739

3.  Design Strategy for the EPR Tumor-Targeting of 1,2-Bis(sulfonyl)-1-alkylhydrazines.

Authors:  Philip G Penketh; Hugh S Williamson; Raymond P Baumann; Krishnamurthy Shyam
Journal:  Molecules       Date:  2021-01-06       Impact factor: 4.411

4.  Influence of glutathione and glutathione S-transferases on DNA interstrand cross-link formation by 1,2-bis(methylsulfonyl)-1-(2-chloroethyl)hydrazine, the active anticancer moiety generated by laromustine.

Authors:  Philip G Penketh; Eric Patridge; Krishnamurthy Shyam; Raymond P Baumann; Rui Zhu; Kimiko Ishiguro; Alan C Sartorelli
Journal:  Chem Res Toxicol       Date:  2014-07-17       Impact factor: 3.739
  4 in total

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