Literature DB >> 86569

Fluorescence flow analysis of lymphocyte activation using Hoechst 33342 dye.

M E Lalande, R G Miller.   

Abstract

The in vitro response of murine lymphocytes to allogeneic and mitogenic stimulation has been studied by using the nontoxic fluorescent DNA probe, Hoechst 33342, and a fluorescence flow cytometer-cell sorter. Under appropriate conditions, two peaks of fluorescent intensity, not related to cellular DNA content, can be seen. As early as 12 hr after culture set up, lymphocyte activation can be identified. It appears that Hoechst-labeled lymphocytes of higher fluorescence intensity represent those cells activated by allogeneic stimulation whereas cells obtained from the lower intensity peak are nonresponding lymphocytes.

Entities:  

Mesh：

Substances：

Year:  1979        PMID： 86569     DOI： 10.1177/27.1.86569

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN： 0022-1554            Impact factor:   2.479


  8 in total

1.  The DNA content of mouse two-cell embryos can be measured by microfluorimetric image analysis under conditions of cell viability.

Authors:  P G Adenot; E Corteggiani; M Geze; D Besombes; P Debey
Journal:  J Fluoresc       Date:  1992-09       Impact factor: 2.217

Review 2.  Cervical cancer stem cells: opportunities and challenges.

Authors:  Ravindresh Chhabra
Journal:  J Cancer Res Clin Oncol       Date:  2015-01-07       Impact factor: 4.553

3.  Karyological studies on established mosquito cell lines.

Authors:  J M Chiplonkar; U V Wagh; G P Phondke; D S Joshi
Journal:  Experientia       Date:  1983-06-15

Review 4.  Fluorescent probes to detect lymphocyte activation.

Authors:  R C Nairn; J M Rolland
Journal:  Clin Exp Immunol       Date:  1980-01       Impact factor: 4.330

5.  Intranuclear differences in the response of Purkinje cell DNA of the rat cerebellum to bleomycin. A microphotometric and autoradiographic study.

Authors:  E Scherini; V Mares; G Bernocchi; S Barni
Journal:  Histochemistry       Date:  1988

6.  Hoechst 33342 dye uptake as a probe of membrane permeability changes in mammalian cells.

Authors:  M E Lalande; V Ling; R G Miller
Journal:  Proc Natl Acad Sci U S A       Date:  1981-01       Impact factor: 11.205

7.  Quantitative studies on the precursors of cytotoxic lymphocytes. VI. Second signal requirements of specifically activated precursors isolated 12 h after stimulation.

Authors:  M E Lalande; M J McCutcheon; R G Miller
Journal:  J Exp Med       Date:  1980-01-01       Impact factor: 14.307

8.  Comparison of fluorescence intensity of Hoechst 33342-stained EMT6 tumour cells and tumour-infiltrating host cells.

Authors:  D A Loeffler; P C Keng; K M Wilson; E M Lord
Journal:  Br J Cancer       Date:  1987-11       Impact factor: 7.640
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.