Literature DB >> 8655527

Site-directed mutational analysis of the osmotically regulated proU promoter of Salmonella typhimurium.

X Zhang1, S A Fletcher, L N Csonka.   

Abstract

We carried out PCR mutagenesis of the proU promoter of Salmonella typhimurium, in order to identify sequences important for its osmotic control. We obtained five mutations in the -35 element: two decreased the promoter strength, one increased it, and the others had no effect. However, none abolished osmotic control, suggesting that the sequence of the -35 element is not crucial for osmotic control.

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Year:  1996        PMID： 8655527      PMCID： PMC178099          DOI： 10.1128/jb.178.11.3377-3379.1996

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  11 in total

1.  Nucleotide sequence of the osmoregulatory proU operon of Escherichia coli.

Authors:  J Gowrishankar
Journal:  J Bacteriol       Date:  1989-04       Impact factor: 3.490

2.  A transcriptional silencer downstream of the promoter in the osmotically controlled proU operon of Salmonella typhimurium.

Authors:  D G Overdier; L N Csonka
Journal:  Proc Natl Acad Sci U S A       Date:  1992-04-01       Impact factor: 11.205

3.  Osmotic control of proU transcription is mediated through direct action of potassium glutamate on the transcription complex.

Authors:  W S Prince; M R Villarejo
Journal:  J Biol Chem       Date:  1990-10-15       Impact factor: 5.157

4.  A physiological role for DNA supercoiling in the osmotic regulation of gene expression in S. typhimurium and E. coli.

Authors:  C F Higgins; C J Dorman; D A Stirling; L Waddell; I R Booth; G May; E Bremer
Journal:  Cell       Date:  1988-02-26       Impact factor: 41.582

5.  Fine-structure deletion analysis of the transcriptional silencer of the proU operon of Salmonella typhimurium.

Authors:  S A Fletcher; L N Csonka
Journal:  J Bacteriol       Date:  1995-08       Impact factor: 3.490

6.  The Escherichia coli proU promoter element and its contribution to osmotically signaled transcription activation.

Authors:  J Mellies; R Brems; M Villarejo
Journal:  J Bacteriol       Date:  1994-06       Impact factor: 3.490

7.  Nucleotide sequence of the transcriptional control region of the osmotically regulated proU operon of Salmonella typhimurium and identification of the 5' endpoint of the proU mRNA.

Authors:  D G Overdier; E R Olson; B D Erickson; M M Ederer; L N Csonka
Journal:  J Bacteriol       Date:  1989-09       Impact factor: 3.490

8.  Molecular characterization of the proU loci of Salmonella typhimurium and Escherichia coli encoding osmoregulated glycine betaine transport systems.

Authors:  D A Stirling; C S Hulton; L Waddell; S F Park; G S Stewart; I R Booth; C F Higgins
Journal:  Mol Microbiol       Date:  1989-08       Impact factor: 3.501

9.  DNA twist, flexibility and transcription of the osmoregulated proU promoter of Salmonella typhimurium.

Authors:  B J Jordi; T A Owen-Hughes; C S Hulton; C F Higgins
Journal:  EMBO J       Date:  1995-11-15       Impact factor: 11.598

10.  The Escherichia coli nucleoid protein H-NS functions directly as a transcriptional repressor.

Authors:  C Ueguchi; T Mizuno
Journal:  EMBO J       Date:  1993-03       Impact factor: 11.598
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  6 in total

1.  Evidence for transcription attenuation rendering cryptic a sigmaS-dependent promoter of the osmotically regulated proU operon of Salmonella typhimurium.

Authors:  K Rajkumari; A Ishihama; J Gowrishankar
Journal:  J Bacteriol       Date:  1997-11       Impact factor: 3.490

2.  In vivo expression from the RpoS-dependent P1 promoter of the osmotically regulated proU operon in Escherichia coli and Salmonella enterica serovar Typhimurium: activation by rho and hns mutations and by cold stress.

Authors:  K Rajkumari; J Gowrishankar
Journal:  J Bacteriol       Date:  2001-11       Impact factor: 3.490

3.  Superimposition of tyrR protein-mediated regulation on osmoresponsive transcription of Escherichia coli proU in vivo.

Authors:  J Gowrishankar; A J Pittard
Journal:  J Bacteriol       Date:  1998-12       Impact factor: 3.490

4.  Osmotic control of opuA expression in Bacillus subtilis and its modulation in response to intracellular glycine betaine and proline pools.

Authors:  Tamara Hoffmann; Annette Wensing; Margot Brosius; Leif Steil; Uwe Völker; Erhard Bremer
Journal:  J Bacteriol       Date:  2012-11-21       Impact factor: 3.490

5.  A single point mutation in the listerial betL σ(A)-dependent promoter leads to improved osmo- and chill-tolerance and a morphological shift at elevated osmolarity.

Authors:  Roland F Hoffmann; Susan McLernon; Audrey Feeney; Colin Hill; Roy D Sleator
Journal:  Bioengineered       Date:  2013-03-11       Impact factor: 3.269

6.  Independent bottlenecks characterize colonization of systemic compartments and gut lymphoid tissue by salmonella.

Authors:  Chee Han Lim; Sabrina Voedisch; Benjamin Wahl; Syed Fazle Rouf; Robert Geffers; Mikael Rhen; Oliver Pabst
Journal:  PLoS Pathog       Date:  2014-07-31       Impact factor: 6.823
  6 in total

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