Literature DB >> 8654990

BstF5I, an unusual isoschizomer of FokI.

M A Abdurashitov1, E V Kileva, N M Shinkarenko, A V Shevchenko, V S Dedkov, S Kh Degtyarev.   

Abstract

BstF5I, a new restriction endonuclease (ENase) from Bacillus stearothermophilus F5, has been discovered. This enzyme recognizes 5'-GGATG-3' and cleaves DNA, generating a 2-base 3'extension: 5'-GGATG NN[symbol: see text]-3' 3'-CCTAC[symbol: see text]NN-5' BstF5I is an isoschizomer of FokI and seems to be evolutionarily close to other nonpalindromic-recognizing ENases from thermophilic bacilli.

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Year:  1996        PMID： 8654990     DOI： 10.1016/0378-1119(96)85083-9

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  2 in total

1.  BtrI, a novel restriction endonuclease, recognises the non-palindromic sequence 5'-CACGTC(-3/-3)-3'.

Authors:  S K Degtyarev; O A Belichenko; N A Lebedeva; V S Dedkov; M A Abdurashitov
Journal:  Nucleic Acids Res       Date:  2000-06-01       Impact factor: 16.971

2.  BstAPI, an ApaBI isoschizomer, cleaves DNA at 5'-GCANNNN NTGC-3'.

Authors:  M A Abdurashitov; O A Belichenko; A V Shevchenko; V S Dedkov; S K Degtyarev
Journal:  Nucleic Acids Res       Date:  1997-06-15       Impact factor: 16.971
  2 in total

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