Literature DB >> 8654578

Heat-labile uracil-DNA glycosylase: purification and characterization.

H Sobek1, M Schmidt, B Frey, K Kaluza.   

Abstract

A uracil-DNA glycosylase (UNG) from a psychrophilic marine bacterium (BMTU 3346) has been purified to apparent homogeneity. The enzyme has a molecular weight of 23400 Da. It is stable in complex buffers (containing glycerol/BSA), whereas it is heat-labile in dilute buffers (free of stabilizers) with a half-life of 2 min at 40 degrees C. Due to the thermolability, uracil-DNA glycosylase is suitable for application in the carryover prevention technique showing less residual activity and/or a slower reactivation rate than the usually applied UNG from Escherichia coli.

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Year:  1996        PMID： 8654578     DOI： 10.1016/0014-5793(96)00444-9

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  9 in total

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Journal:  Virol J       Date:  2005-04-11       Impact factor: 4.099

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  9 in total

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