Literature DB >> 8648747

Synthesis of Semliki Forest virus RNA polymerase components nsP1 through nsP4 in Saccharomyces cerevisiae by expression of cDNA encoding the nonstructural polyprotein.

P Russo1, P Laakkonen, T Ahola, L Kääriäinen.   

Abstract

A Semliki Forest virus nonstructural polyprotein, P1234, expressed in the yeast Saccharomyces cerevisiae in the absence of a replicative RNA template appeared to be properly cleaved into nsP1 to nsP4. All nsPs were membrane associated, and nsP2 was also transported to the nucleus. The membrane fraction containing nsPs showed guanine-7-methyltransferase and guanylyltransferase-like activities, typical for Semliki Forest virus nsP1.

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Year:  1996        PMID： 8648747      PMCID： PMC190290     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  24 in total

1.  Nuclear localization of Semliki Forest virus-specific nonstructural protein nsP2.

Authors:  J Peränen; M Rikkonen; P Liljeström; L Kääriäinen
Journal:  J Virol       Date:  1990-05       Impact factor: 5.103

2.  The alphavirus replicase protein nsP1 is membrane-associated and has affinity to endocytic organelles.

Authors:  J Peränen; P Laakkonen; M Hyvönen; L Kääriäinen
Journal:  Virology       Date:  1995-04-20       Impact factor: 3.616

3.  Replication of the genome of alphaviruses.

Authors:  L Kääriäinen; K Takkinen; S Keränen; H Söderlund
Journal:  J Cell Sci Suppl       Date:  1987

4.  Transcription terminates near the poly(A) site in the CYC1 gene of the yeast Saccharomyces cerevisiae.

Authors:  P Russo; F Sherman
Journal:  Proc Natl Acad Sci U S A       Date:  1989-11       Impact factor: 11.205

5.  Solubilization and immunoprecipitation of alphavirus replication complexes.

Authors:  D J Barton; S G Sawicki; D L Sawicki
Journal:  J Virol       Date:  1991-03       Impact factor: 5.103

6.  DNA sequence required for efficient transcription termination in yeast.

Authors:  K S Zaret; F Sherman
Journal:  Cell       Date:  1982-03       Impact factor: 41.582

7.  Demonstration in vitro of temperature-sensitive elongation of RNA in Sindbis virus mutant ts6.

Authors:  D J Barton; S G Sawicki; D L Sawicki
Journal:  J Virol       Date:  1988-10       Impact factor: 5.103

8.  Biogenesis of type I cytopathic vacuoles in Semliki Forest virus-infected BHK cells.

Authors:  J Peränen; L Kääriäinen
Journal:  J Virol       Date:  1991-03       Impact factor: 5.103

9.  Semliki Forest virus-specific non-structural protein nsP3 is a phosphoprotein.

Authors:  J Peränen; K Takkinen; N Kalkkinen; L Kääriäinen
Journal:  J Gen Virol       Date:  1988-09       Impact factor: 3.891

10.  Alphavirus RNA replicase is located on the cytoplasmic surface of endosomes and lysosomes.

Authors:  S Froshauer; J Kartenbeck; A Helenius
Journal:  J Cell Biol       Date:  1988-12       Impact factor: 10.539
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  2 in total

1.  Characterization of the AdoMet-dependent guanylyltransferase activity that is associated with the N terminus of bamboo mosaic virus replicase.

Authors:  Y I Li; Y J Chen; Y H Hsu; M Meng
Journal:  J Virol       Date:  2001-01       Impact factor: 5.103

Review 2.  Functions of alphavirus nonstructural proteins in RNA replication.

Authors:  Leevi Kääriäinen; Tero Ahola
Journal:  Prog Nucleic Acid Res Mol Biol       Date:  2002
  2 in total

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