Literature DB >> 864838

Sequence analysis of the nicks and termini of bacteriophage T5 DNA.

B P Nichols, J E Donelson.   

Abstract

Bacteriophage T5 DNA, when isolated from mature phage particles, contains several nicks in one of the two strands. The 5'-terminal nucleotides at the nicks were labeled with polynucleotide kinase and [gamma-32P]ATP, and the 3'-terminal nucleotides were labeled with Escherichia coli DNA polymerase I and [alpha-32P]dGTP. The sequences around the nicks were analyzed by partial nuclease digestion followed by homochromatography fractionation of the resulting oligonucleotides. The nicks had at least the sequence -PuOH pGpCpGpC- in common. In addition, the two 5' external termini had the first seven nucleotides in common.

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Year:  1977        PMID： 864838      PMCID： PMC515742     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  15 in total

1.  Bacteriophage T5-induced endonucleases that introduce site-specific single-chain interruptions in duplex DNA.

Authors:  S G Rogers; M Rhoades
Journal:  Proc Natl Acad Sci U S A       Date:  1976-05       Impact factor: 11.205

2.  Terminal repetition in the DNA of bacteriophage T5.

Authors:  M Rhoades; E A Rhoades
Journal:  J Mol Biol       Date:  1972-08-21       Impact factor: 5.469

3.  Determination of a nucleotide sequence in bacteriophage f1 DNA by primed synthesis with DNA polymerase.

Authors:  F Sanger; J E Donelson; A R Coulson; H Kössel; D Fischer
Journal:  J Mol Biol       Date:  1974-12-05       Impact factor: 5.469

4.  Fractionation and sequences of the large pyrimidine oligonucleotides from bacteriophage fd DNA.

Authors:  V Ling
Journal:  J Mol Biol       Date:  1972-02-28       Impact factor: 5.469

5.  Active center of DNA polymerase.

Authors:  A Kornberg
Journal:  Science       Date:  1969-03-28       Impact factor: 47.728

6.  Unique double-stranded fragments of bacteriophage T5 DNA resulting from preferential shear-induced breakage at nicks.

Authors:  G S Hayward
Journal:  Proc Natl Acad Sci U S A       Date:  1974-05       Impact factor: 11.205

7.  The synthesis and purification of (gamma-32P)-adenosine triphosphate with high specific activity.

Authors:  P F Schendel; R D Wells
Journal:  J Biol Chem       Date:  1973-12-10       Impact factor: 5.157

8.  The chromosome of bacteriophage T5. II. Arrangement of the single-stranded DNA fragments in the T5 + and T5st(O) chromosomes.

Authors:  G S Hayward; M G Smith
Journal:  J Mol Biol       Date:  1972-02-14       Impact factor: 5.469

9.  The chromosome of bacteriophage T5. I. Analysis of the single-stranded DNA fragments by agarose gel electrophoresis.

Authors:  G S Hayward; M G Smith
Journal:  J Mol Biol       Date:  1972-02-14       Impact factor: 5.469

10.  Distribution of "minor" nicks in bacteriophage T5 DNA.

Authors:  J V Johnston; B P Nichols; J E Donelson
Journal:  J Virol       Date:  1977-05       Impact factor: 5.103
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  5 in total

1.  Interruption-deficient mutants of bacteriophage T5: analysis of single-site mutants.

Authors:  M Rhoades
Journal:  J Virol       Date:  1986-08       Impact factor: 5.103

2.  Interruption-deficient mutants of bacteriophage T5 I. Isolation and general properties.

Authors:  S G Rogers; E A Godwin; E S Shinosky; M Rhoades
Journal:  J Virol       Date:  1979-02       Impact factor: 5.103

3.  Localization of the dihydrofolate reductase gene on the physical map of bacteriophage T5.

Authors:  G Peter; U J Hänggi; H G Zachau
Journal:  Mol Gen Genet       Date:  1979-10-01

4.  Mapping and cloning of Eco RI-fragments of bacteriophage T5+ DNA.

Authors:  B P Nichols; J E Donelson
Journal:  Nucleic Acids Res       Date:  1977-11       Impact factor: 16.971

5.  Distribution of "minor" nicks in bacteriophage T5 DNA.

Authors:  J V Johnston; B P Nichols; J E Donelson
Journal:  J Virol       Date:  1977-05       Impact factor: 5.103
  5 in total

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