Literature DB >> 8641748

Identification and purification of novel internalin-related proteins in Listeria monocytogenes and Listeria ivanovii.

A Lingnau1, T Chakraborty, K Niebuhr, E Domann, J Wehland.   

Abstract

Monoclonal antibodies were generated against a 30-kDa protein fraction derived from culture supernatants of a Listeria monocytogenes strain complemented with additional copies of the prfA regulator gene. Several of the antibodies reacted specifically with a hitherto unidentified, secreted 30-kDa polypeptide. By immunoblot analysis, the expression of this 30kDa polypeptide was found to be dependent on the presence of the PrfA regulator protein. Microsequencing of peptides derived from the partially purified 30-kDa protein revealed homologies to the InlA and InlB polypeptides of L. monocytogenes, which are required for the internalization of the bacteria into nonphagocytic cell lines. This prompted us to term the 30-kDa polypeptide internalin-related protein (Irp). Irp-specific monoclonal antibodies cross-reacted with a 24-kDa polypeptide present in culture supernatants of Listeria ivanovii, indicating the existence of an Irp-related protein in this pathogenic Listeria species.

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Year:  1996        PMID： 8641748      PMCID： PMC173869          DOI： 10.1128/iai.64.3.1002-1006.1996

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  23 in total

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Authors:  A Rüdiger; M Rüdiger; K Weber; D Schomburg
Journal:  Anal Biochem       Date:  1995-01-20       Impact factor: 3.365

2.  Coordinate regulation of virulence genes in Listeria monocytogenes requires the product of the prfA gene.

Authors:  T Chakraborty; M Leimeister-Wächter; E Domann; M Hartl; W Goebel; T Nichterlein; S Notermans
Journal:  J Bacteriol       Date:  1992-01       Impact factor: 3.490

3.  An agarose-based gel-concentration system for microsequence and mass spectrometric characterization of proteins previously purified in polyacrylamide gels starting at low picomole levels.

Authors:  M H Rider; M Puype; J Van Damme; K Gevaert; S De Boeck; J D'Alayer; H H Rasmussen; J E Celis; J Vanderkerchove
Journal:  Eur J Biochem       Date:  1995-05-15

Review 4.  Molecular and genetic determinants of the Listeria monocytogenes infectious process.

Authors:  B Sheehan; C Kocks; S Dramsi; E Gouin; A D Klarsfeld; J Mengaud; P Cossart
Journal:  Curr Top Microbiol Immunol       Date:  1994       Impact factor: 4.291

5.  Common features of gram-positive bacterial proteins involved in cell recognition.

Authors:  S Dramsi; P Dehoux; P Cossart
Journal:  Mol Microbiol       Date:  1993-09       Impact factor: 3.501

Review 6.  Listeria monocytogenes, a food-borne pathogen.

Authors:  J M Farber; P I Peterkin
Journal:  Microbiol Rev       Date:  1991-09

7.  Entry of L. monocytogenes into cells is mediated by internalin, a repeat protein reminiscent of surface antigens from gram-positive cocci.

Authors:  J L Gaillard; P Berche; C Frehel; E Gouin; P Cossart
Journal:  Cell       Date:  1991-06-28       Impact factor: 41.582

8.  Nucleotide sequence of the lecithinase operon of Listeria monocytogenes and possible role of lecithinase in cell-to-cell spread.

Authors:  J A Vazquez-Boland; C Kocks; S Dramsi; H Ohayon; C Geoffroy; J Mengaud; P Cossart
Journal:  Infect Immun       Date:  1992-01       Impact factor: 3.441

9.  Entry of Listeria monocytogenes into hepatocytes requires expression of inIB, a surface protein of the internalin multigene family.

Authors:  S Dramsi; I Biswas; E Maguin; L Braun; P Mastroeni; P Cossart
Journal:  Mol Microbiol       Date:  1995-04       Impact factor: 3.501

10.  Identification and structural characterization of a mannose-6-phosphate containing oligomannosidic N-glycan from human erythropoietin secreted by recombinant BHK-21 cells.

Authors:  M Nimtz; V Wray; A Rüdiger; H S Conradt
Journal:  FEBS Lett       Date:  1995-05-29       Impact factor: 4.124
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  14 in total

1.  Variations in the Nanomechanical Properties of Virulent and Avirulent Listeria monocytogenes.

Authors:  Bong-Jae Park; Nehal I Abu-Lail
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Review 2.  Listeria pathogenesis and molecular virulence determinants.

Authors:  J A Vázquez-Boland; M Kuhn; P Berche; T Chakraborty; G Domínguez-Bernal; W Goebel; B González-Zorn; J Wehland; J Kreft
Journal:  Clin Microbiol Rev       Date:  2001-07       Impact factor: 26.132

Review 3.  Short-sequence DNA repeats in prokaryotic genomes.

Authors:  A van Belkum; S Scherer; L van Alphen; H Verbrugh
Journal:  Microbiol Mol Biol Rev       Date:  1998-06       Impact factor: 11.056

4.  Intracellular gene expression profile of Listeria monocytogenes.

Authors:  Som Subhra Chatterjee; Hamid Hossain; Sonja Otten; Carsten Kuenne; Katja Kuchmina; Silke Machata; Eugen Domann; Trinad Chakraborty; Torsten Hain
Journal:  Infect Immun       Date:  2006-02       Impact factor: 3.441

5.  Identification, cloning, and characterization of the Ima operon, whose gene products are unique to Listeria monocytogenes.

Authors:  S Schäferkordt; T Chakraborty
Journal:  J Bacteriol       Date:  1997-04       Impact factor: 3.490

6.  Listeriolysin and IrpA are major protein targets of the human humoral response against Listeria monocytogenes.

Authors:  R Grenningloh; A Darji; J Wehland; T Chakraborty; S Weiss
Journal:  Infect Immun       Date:  1997-09       Impact factor: 3.441

7.  The Listeria monocytogenes InlC protein interferes with innate immune responses by targeting the I{kappa}B kinase subunit IKK{alpha}.

Authors:  Edith Gouin; Minou Adib-Conquy; Damien Balestrino; Marie-Anne Nahori; Véronique Villiers; Frédéric Colland; Shaynoor Dramsi; Olivier Dussurget; Pascale Cossart
Journal:  Proc Natl Acad Sci U S A       Date:  2010-09-20       Impact factor: 11.205

8.  Identification and characterization of a novel PrfA-regulated gene in Listeria monocytogenes whose product, IrpA, is highly homologous to internalin proteins, which contain leucine-rich repeats.

Authors:  E Domann; S Zechel; A Lingnau; T Hain; A Darji; T Nichterlein; J Wehland; T Chakraborty
Journal:  Infect Immun       Date:  1997-01       Impact factor: 3.441

9.  Identification of four new members of the internalin multigene family of Listeria monocytogenes EGD.

Authors:  S Dramsi; P Dehoux; M Lebrun; P L Goossens; P Cossart
Journal:  Infect Immun       Date:  1997-05       Impact factor: 3.441

Review 10.  Surface proteins of gram-positive bacteria and mechanisms of their targeting to the cell wall envelope.

Authors:  W W Navarre; O Schneewind
Journal:  Microbiol Mol Biol Rev       Date:  1999-03       Impact factor: 11.056
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