| Literature DB >> 863983 |
J C Loo, A G Butterfield, J Moffatt, N Jordan.
Abstract
A sensitive, specific high-performance liquid chromatographic procedure for the determination of prednisolone in plasma is described. The organic solvent extract from plasma is chromatographed on a silica gel column using a mobile phase of 0.2% glacial acetic acid, 6% ethanol, 30% methylene chloride in n-hexane on a high-performance liquid chromatograph fitted with an ultraviolet dector (254 nm). Quantitation of plasma samples containing 25 ng/ml prednisolone is reported. Metabolites and endogenous hydrocortisone do not interfere with prednisolone. The determination of prednisolone concentration in plasma following administration of a 10-mg single oral dose to a human subject is described.Entities:
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Year: 1977 PMID： 863983 DOI： 10.1016/s0378-4347(00)81807-1
Source DB: PubMed Journal: J Chromatogr