Literature DB >> 8639741

Cloning and characterization of an Arabidopsis thaliana cDNA clone encoding an organellar isoform of serine acetyltransferase.

M A Roberts1, J L Wray.   

Abstract

We have cloned an Arabidopsis thaliana cDNA encoding serine acetyltransferase (EC 2.3.1.30) by functional complementation of the Escherichia coli cysE mutant JM15. The cDNA clone Sat-1 conferred serine acetyltransferase activity (with apparent Km for the two substrates acetyl CoA and L-serine of 0.043 and 3.47 mmol/dm3 respectively) on the cysE mutant. The 1515 bp full-length cDNA encodes a deduced protein of 391 amino acids which includes a putative chloroplastic targeting presequence. Northern analysis revealed a single message of 1.5 kb, while Southern hybridisation suggests a small multigene family of related sequences.

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Year:  1996        PMID： 8639741     DOI： 10.1007/bf00020814

Source DB:  PubMed          Journal:  Plant Mol Biol        ISSN： 0167-4412            Impact factor:   4.076


  43 in total

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Journal:  Genetics       Date:  1988-12       Impact factor: 4.562

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7.  Subcellular distribution of serine acetyltransferase from Pisum sativum and characterization of an Arabidopsis thaliana putative cytosolic isoform.

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Journal:  Eur J Biochem       Date:  1995-01-15

8.  Modulation of cysteine biosynthesis in chloroplasts of transgenic tobacco overexpressing cysteine synthase [O-acetylserine(thiol)-lyase].

Authors:  K Saito; M Kurosawa; K Tatsuguchi; Y Takagi; I Murakoshi
Journal:  Plant Physiol       Date:  1994-11       Impact factor: 8.340

9.  Cysteine biosynthesis in plants: isolation and functional identification of a cDNA encoding a serine acetyltransferase from Arabidopsis thaliana.

Authors:  N Bogdanova; C Bork; R Hell
Journal:  FEBS Lett       Date:  1995-01-16       Impact factor: 4.124

10.  Selection of AUG initiation codons differs in plants and animals.

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Journal:  EMBO J       Date:  1987-01       Impact factor: 11.598
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  6 in total

1.  Sulfate metabolism.

Authors:  Thomas Leustek
Journal:  Arabidopsis Book       Date:  2002-04-04

2.  Regulation of sulfur assimilation in higher plants: a sulfate transporter induced in sulfate-starved roots plays a central role in Arabidopsis thaliana.

Authors:  H Takahashi; M Yamazaki; N Sasakura; A Watanabe; T Leustek; J A Engler; G Engler; M Van Montagu; K Saito
Journal:  Proc Natl Acad Sci U S A       Date:  1997-09-30       Impact factor: 11.205

3.  Characterization and expression analysis of a serine acetyltransferase gene family involved in a key step of the sulfur assimilation pathway in Arabidopsis.

Authors:  Cintia Goulart Kawashima; Oliver Berkowitz; Ruediger Hell; Masaaki Noji; Kazuki Saito
Journal:  Plant Physiol       Date:  2004-12-03       Impact factor: 8.340

4.  Structure of soybean serine acetyltransferase and formation of the cysteine regulatory complex as a molecular chaperone.

Authors:  Hankuil Yi; Sanghamitra Dey; Sangaralingam Kumaran; Soon Goo Lee; Hari B Krishnan; Joseph M Jez
Journal:  J Biol Chem       Date:  2013-11-13       Impact factor: 5.157

5.  The serine acetyltransferase gene family in Arabidopsis thaliana and the regulation of its expression by cadmium.

Authors:  Jonathan R Howarth; José R Domínguez-Solís; Gloria Gutiérrez-Alcalá; John L Wray; Luis C Romero; Cecilia Gotor
Journal:  Plant Mol Biol       Date:  2003-03       Impact factor: 4.076

6.  Sulfur assimilation in soybean ( Glycine max [L.] Merr.): molecular cloning and characterization of a cytosolic isoform of serine acetyltransferase.

Authors:  Demosthenis Chronis; Hari B Krishnan
Journal:  Planta       Date:  2003-10-30       Impact factor: 4.116
  6 in total

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