Literature DB >> 8637873

Genetic evidence that the RAG1 protein directly participates in V(D)J recombination through substrate recognition.

C A Roman1, D Baltimore.   

Abstract

RAG1 protein is essential for the activation of V(D)J recombination in developing lymphocytes (V, variable; D, diversity; J, joining). However, it has not been determined whether its role involves substrate recognition and catalysis. A single amino acid substitution mutation in the RAG1 gene has now been identified that renders its activity sensitive to the sequence of the coding region abutting the heptamer site in the recombination signal sequence. These results strongly imply that RAG1 interacts directly with DNA.

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Year:  1996        PMID： 8637873      PMCID： PMC39796          DOI： 10.1073/pnas.93.6.2333

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  28 in total

1.  A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions.

Authors:  R Higuchi; B Krummel; R K Saiki
Journal:  Nucleic Acids Res       Date:  1988-08-11       Impact factor: 16.971

2.  Stable expression of immunoglobulin gene V(D)J recombinase activity by gene transfer into 3T3 fibroblasts.

Authors:  D G Schatz; D Baltimore
Journal:  Cell       Date:  1988-04-08       Impact factor: 41.582

3.  V(D)J recombination: a functional definition of the joining signals.

Authors:  J E Hesse; M R Lieber; K Mizuuchi; M Gellert
Journal:  Genes Dev       Date:  1989-07       Impact factor: 11.361

4.  Selective extraction of polyoma DNA from infected mouse cell cultures.

Authors:  B Hirt
Journal:  J Mol Biol       Date:  1967-06-14       Impact factor: 5.469

5.  Extrachromosomal DNA substrates in pre-B cells undergo inversion or deletion at immunoglobulin V-(D)-J joining signals.

Authors:  J E Hesse; M R Lieber; M Gellert; K Mizuuchi
Journal:  Cell       Date:  1987-06-19       Impact factor: 41.582

6.  Expression and V(D)J recombination activity of mutated RAG-1 proteins.

Authors:  M J Sadofsky; J E Hesse; J F McBlane; M Gellert
Journal:  Nucleic Acids Res       Date:  1993-12-11       Impact factor: 16.971

7.  Characterization of broken DNA molecules associated with V(D)J recombination.

Authors:  D B Roth; C Zhu; M Gellert
Journal:  Proc Natl Acad Sci U S A       Date:  1993-11-15       Impact factor: 11.205

8.  Double-strand signal sequence breaks in V(D)J recombination are blunt, 5'-phosphorylated, RAG-dependent, and cell cycle regulated.

Authors:  M Schlissel; A Constantinescu; T Morrow; M Baxter; A Peng
Journal:  Genes Dev       Date:  1993-12       Impact factor: 11.361

9.  Increased frequency of N-region insertion in a murine pre-B-cell line infected with a terminal deoxynucleotidyl transferase retroviral expression vector.

Authors:  N R Landau; D G Schatz; M Rosa; D Baltimore
Journal:  Mol Cell Biol       Date:  1987-09       Impact factor: 4.272

10.  The defect in murine severe combined immune deficiency: joining of signal sequences but not coding segments in V(D)J recombination.

Authors:  M R Lieber; J E Hesse; S Lewis; G C Bosma; N Rosenberg; K Mizuuchi; M J Bosma; M Gellert
Journal:  Cell       Date:  1988-10-07       Impact factor: 41.582
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  15 in total

1.  Conditional RAG-1 mutants block the hairpin formation step of V(D)J recombination.

Authors:  S B Kale; M A Landree; D B Roth
Journal:  Mol Cell Biol       Date:  2001-01       Impact factor: 4.272

Review 2.  The RAG proteins in V(D)J recombination: more than just a nuclease.

Authors:  M J Sadofsky
Journal:  Nucleic Acids Res       Date:  2001-04-01       Impact factor: 16.971

3.  Roles of the "dispensable" portions of RAG-1 and RAG-2 in V(D)J recombination.

Authors:  S B Steen; J O Han; C Mundy; M A Oettinger; D B Roth
Journal:  Mol Cell Biol       Date:  1999-04       Impact factor: 4.272

4.  Detection of RAG protein-V(D)J recombination signal interactions near the site of DNA cleavage by UV cross-linking.

Authors:  Q M Eastman; I J Villey; D G Schatz
Journal:  Mol Cell Biol       Date:  1999-05       Impact factor: 4.272

5.  Mutational analysis of all conserved basic amino acids in RAG-1 reveals catalytic, step arrest, and joining-deficient mutants in the V(D)J recombinase.

Authors:  Leslie E Huye; Mary M Purugganan; Ming-Ming Jiang; David B Roth
Journal:  Mol Cell Biol       Date:  2002-05       Impact factor: 4.272

6.  V(D)J recombination: in vitro coding joint formation.

Authors:  F Weis-Garcia; E Besmer; D J Sawchuk; W Yu; Y Hu; S Cassard; M C Nussenzweig; P Cortes
Journal:  Mol Cell Biol       Date:  1997-11       Impact factor: 4.272

7.  V(D)J recombination frequency is affected by the sequence interposed between a pair of recombination signals: sequence comparison reveals a putative recombinational enhancer element.

Authors:  F A Roch; R Hobi; M W Berchtold; C C Kuenzle
Journal:  Nucleic Acids Res       Date:  1997-06-15       Impact factor: 16.971

8.  Omenn's Syndrome: A rare primary immunodeficiency disorder.

Authors:  Ibtisam B Elnour; Shakeel Ahmed; Kamal Halim; V Nirmala
Journal:  Sultan Qaboos Univ Med J       Date:  2007-08

9.  RAG-2 promotes heptamer occupancy by RAG-1 in the assembly of a V(D)J initiation complex.

Authors:  P C Swanson; S Desiderio
Journal:  Mol Cell Biol       Date:  1999-05       Impact factor: 4.272

10.  A RAG1 mutation found in Omenn syndrome causes coding flank hypersensitivity: a novel mechanism for antigen receptor repertoire restriction.

Authors:  Serre-Yu Wong; Catherine P Lu; David B Roth
Journal:  J Immunol       Date:  2008-09-15       Impact factor: 5.422
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