Literature DB >> 8636376

Characterization of monoclonal antibodies specific for the human growth hormone 22K and 20K isoforms.

M Mellado1, J M Rodriguez-Frade, L Kremer, C Martinez-Alonso.   

Abstract

We have derived and characterized a set of monoclonal antibodies (mAb) specific for the different human GH (hGH) isoforms. The binding characteristics of each antibody to the hGH isoforms (22K and 20K) were analyzed in direct and competitive immunoassays as well as by Western blot. We studied the effects of these mAb on the biological activity of hGH and showed that they specifically block their respective activities. Using these mAb, we developed several immunoassays that have been applied for the quantitation of the different hGH isoforms in body fluids. Therefore, these mAb may help to unravel the biological function of these variants.

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Year:  1996        PMID： 8636376     DOI： 10.1210/jcem.81.4.8636376

Source DB:  PubMed          Journal:  J Clin Endocrinol Metab        ISSN： 0021-972X            Impact factor:   5.958


  4 in total

Review 1.  Molecular heterogeneity of human GH: from basic research to clinical implications.

Authors:  C L Boguszewski
Journal:  J Endocrinol Invest       Date:  2003-03       Impact factor: 4.256

2.  Evaluation of growth hormone bioactivity using the Nb2 cell bioassay in children with growth disorders.

Authors:  M Bozzola; M Zecca; F Locatelli; G Radetti; S Pagani; M Autelli; L Tatò; P Chatelain
Journal:  J Endocrinol Invest       Date:  1998-12       Impact factor: 4.256

3.  Repetitive stimulation of the pituitary with growth-hormone-releasing hormone alters the proportion of 22 and 20 kilodalton human-growth hormone released.

Authors:  Emma A Webb; P Jane Pringle; Iain C A F Robinson; Peter C Hindmarsh
Journal:  Int J Pediatr Endocrinol       Date:  2010-06-09

4.  Functional inactivation of CXC chemokine receptor 4-mediated responses through SOCS3 up-regulation.

Authors:  Silvia F Soriano; Patricia Hernanz-Falcón; José Miguel Rodríguez-Frade; Ana Martín De Ana; Ruth Garzón; Carla Carvalho-Pinto; Antonio J Vila-Coro; Angel Zaballos; Dimitrios Balomenos; Carlos Martínez-A; Mario Mellado
Journal:  J Exp Med       Date:  2002-08-05       Impact factor: 14.307
  4 in total

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