Literature DB >> 8635733

Selectable cassettes for simplified construction of yeast gene disruption vectors.

M C Earley1, G F Crouse.   

Abstract

Cassettes based on a hisG-URA3-hisG insert have been modified by the addition of an KmR-encoding gene and flanking polylinker sites, greatly simplifying construction of gene disruption vectors in Escherichia coli. After gene disruption in yeast, URA3 can then be excised by recombination between the hisG repeats flanking the gene, permitting reuse of the URA3 marker.

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Year:  1996        PMID： 8635733     DOI： 10.1016/0378-1119(95)00805-5

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  6 in total

1.  The role of mismatch repair in the prevention of base pair mutations in Saccharomyces cerevisiae.

Authors:  M C Earley; G F Crouse
Journal:  Proc Natl Acad Sci U S A       Date:  1998-12-22       Impact factor: 11.205

2.  Regulation of mitotic homeologous recombination in yeast. Functions of mismatch repair and nucleotide excision repair genes.

Authors:  A Nicholson; M Hendrix; S Jinks-Robertson; G F Crouse
Journal:  Genetics       Date:  2000-01       Impact factor: 4.562

3.  Identification of a xylose reductase gene in the xylose metabolic pathway of Kluyveromyces marxianus NBRC1777.

Authors:  Biao Zhang; Ling Zhang; Dongmei Wang; Xiaolian Gao; Jiong Hong
Journal:  J Ind Microbiol Biotechnol       Date:  2011-06-04       Impact factor: 3.346

4.  CgCYN1, a plasma membrane cystine-specific transporter of Candida glabrata with orthologues prevalent among pathogenic yeast and fungi.

Authors:  Amit Kumar Yadav; Anand Kumar Bachhawat
Journal:  J Biol Chem       Date:  2011-04-20       Impact factor: 5.157

5.  The effects of mismatch repair and RAD1 genes on interchromosomal crossover recombination in Saccharomyces cerevisiae.

Authors:  Ainsley Nicholson; Rebecca M Fabbri; Jason W Reeves; Gray F Crouse
Journal:  Genetics       Date:  2006-04-02       Impact factor: 4.562

6.  Mutations in RNA polymerase II and elongation factor SII severely reduce mRNA levels in Saccharomyces cerevisiae.

Authors:  J C Lennon; M Wind; L Saunders; M B Hock; D Reines
Journal:  Mol Cell Biol       Date:  1998-10       Impact factor: 4.272
  6 in total

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