Literature DB >> 8635578

Expression of human inducible nitric oxide synthase in Escherichia coli.

J D Fossetta1, X D Niu, C A Lunn, P J Zavodny, S K Narula, D Lundell.   

Abstract

We have expressed active full-length human inducible nitric oxide synthase (iNOS) in E. coli. Expression required co-expression with calmodulin, a particularly tight-binding cofactor. The extracts also required tetrahydrobiopterin to display activity. Specific activity of the purified recombinant iNOS was similar to iNOS purified from murine macrophages. This result indicates that no special processing events unique to eucaryotic cells are necessary for iNOS activity.

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Year:  1996        PMID： 8635578     DOI： 10.1016/0014-5793(95)01496-9

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  7 in total

1.  Optimization of Blood-Brain Barrier Permeability with Potent and Selective Human Neuronal Nitric Oxide Synthase Inhibitors Having a 2-Aminopyridine Scaffold.

Authors:  Ha T Do; Huiying Li; Georges Chreifi; Thomas L Poulos; Richard B Silverman
Journal:  J Med Chem       Date:  2019-02-25       Impact factor: 7.446

2.  Nitric oxide synthase domain interfaces regulate electron transfer and calmodulin activation.

Authors:  Brian C Smith; Eric S Underbakke; Daniel W Kulp; William R Schief; Michael A Marletta
Journal:  Proc Natl Acad Sci U S A       Date:  2013-09-03       Impact factor: 11.205

3.  Identification of critical amino acid residues for human iNOS functional activity.

Authors:  Saule Naureckiene; Sreekumar R Kodangattil; Edward J Kaftan; Philip G Jones; Jeffrey D Kennedy; Kathryn E Rogers; Pranab K Chanda
Journal:  Protein J       Date:  2008-08       Impact factor: 2.371

4.  GW274150 and GW273629 are potent and highly selective inhibitors of inducible nitric oxide synthase in vitro and in vivo.

Authors:  Wendy K Alderton; Anthony D R Angell; Caroline Craig; John Dawson; Edward Garvey; Salvador Moncada; Jayne Monkhouse; Daryl Rees; Linda J Russell; Rachel J Russell; Sheila Schwartz; Neil Waslidge; Richard G Knowles
Journal:  Br J Pharmacol       Date:  2005-06       Impact factor: 8.739

5.  Effects of transition metals on nitric oxide synthase catalysis.

Authors:  J M Perry; M A Marletta
Journal:  Proc Natl Acad Sci U S A       Date:  1998-09-15       Impact factor: 11.205

Review 6.  Modulation of autoimmune diseases by nitric oxide.

Authors:  V K Singh; S Mehrotra; P Narayan; C M Pandey; S S Agarwal
Journal:  Immunol Res       Date:  2000       Impact factor: 2.829

7.  First Contact: 7-Phenyl-2-Aminoquinolines, Potent and Selective Neuronal Nitric Oxide Synthase Inhibitors That Target an Isoform-Specific Aspartate.

Authors:  Maris A Cinelli; Cory T Reidl; Huiying Li; Georges Chreifi; Thomas L Poulos; Richard B Silverman
Journal:  J Med Chem       Date:  2020-04-17       Impact factor: 7.446
  7 in total

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