| Literature DB >> 8632445 |
M H Moers1, W H Kalle, A G Ruiter, J C Wiegant, A K Raap, J Greve, B G de Grooth, N F van Hulst.
Abstract
Fluorescence in situ hybridization on human metaphase chromosomes is detected by near-field scanning optical microscopy. This combination of cytochemical and scanning probe techniques enables the localization and identification of several fluorescently labelled genomic DNA fragments on a single chromosome with an unprecedented resolution. Three nucleic acid probes are used: pUC1.77, p1-79 and the plasmid probe alpha-spectrin. The hybridization signals are very well resolved in the near-field fluorescence images, while the exact location of the probes can be correlated accurately with the chromosome topography as afforded by the shear force image.Entities:
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Year: 1996 PMID： 8632445 DOI： 10.1111/j.1365-2818.1996.tb04795.x
Source DB: PubMed Journal: J Microsc ISSN： 0022-2720 Impact factor: 1.758