Literature DB >> 8630275

Effects of neocarzinostatin-chimeric Fab conjugates on the growth of human pancreatic carcinoma xenografts.

E Otsuji1, T Yamaguchi, H Tsuruta, Y Yata, H Nishi, K Okamoto, K Taniguchi, M Kato, T Kotani, K Kitamura, T Takahashi.   

Abstract

Neocarzinostatin (NCS) was bound covalently to human/mouse chimeric Fab fragments of MAb A7 (chA7Fab) directed against human pancreatic carcinoma. The anti-tumour effect of chA7Fab-NCS was tested in a nude mouse model on pancreatic carcinoma and compared with A7-NCS or NCS alone. The anti-tumour effect of chA7Fab-NCS increased in a dose-dependent manner and was significantly greater than either A7-NCS or NCS. Tumour growth was completely suppressed after the administration of chA7Fab-NCS. An enzyme-linked immunosorbent assay with rabbit anti-mouse immunoglobulin was performed to examine the antigenicity of chA7Fab. ChA7Fab had less reactivity with rabbit anti-mouse immunoglobulin than either whole antibody A7 or murine Fab fragments of A7. Thus, chA7Fab-NCS can inhibit human pancreatic cancer growth in an animal and may be useful for targeting chemotherapy to pancreatic cancer in humans.

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Year:  1996        PMID： 8630275      PMCID： PMC2074529          DOI： 10.1038/bjc.1996.227

Source DB:  PubMed          Journal:  Br J Cancer        ISSN： 0007-0920            Impact factor:   7.640


  25 in total

1.  Immune function of patients with gastrointestinal carcinoma after treatment with multiple infusions of monoclonal antibody 17.1A.

Authors:  H M Blottière; C Maurel; J Y Douillard
Journal:  Cancer Res       Date:  1987-10-01       Impact factor: 12.701

2.  Specific cytotoxic effect of neocarzinostatin conjugated to monoclonal antibody A7 on human pancreatic carcinoma.

Authors:  E Otsuji; T Takahashi; T Yamaguchi; N Yamaguchi; J Imanishi
Journal:  Gastroenterol Jpn       Date:  1990-04

3.  Antitumor activity of the monoclonal antibody-Vinca alkaloid immunoconjugate LY203725 (KS1/4-4-desacetylvinblastine-3-carboxhydrazide) in a nude mouse model of human ovarian cancer.

Authors:  L D Apelgren; D L Zimmerman; S L Briggs; T F Bumol
Journal:  Cancer Res       Date:  1990-06-15       Impact factor: 12.701

4.  Secretory expression of the human serum albumin gene in the yeast, Saccharomyces cerevisiae.

Authors:  K Okabayashi; Y Nakagawa; N Hayasuke; H Ohi; M Miura; Y Ishida; M Shimizu; K Murakami; K Hirabayashi; H Minamino
Journal:  J Biochem       Date:  1991-07       Impact factor: 3.387

5.  Penetration and binding of radiolabeled anti-carcinoembryonic antigen monoclonal antibodies and their antigen binding fragments in human colon multicellular tumor spheroids.

Authors:  R Sutherland; F Buchegger; M Schreyer; A Vacca; J P Mach
Journal:  Cancer Res       Date:  1987-03-15       Impact factor: 12.701

6.  By-passing immunisation. Human antibodies from synthetic repertoires of germline VH gene segments rearranged in vitro.

Authors:  H R Hoogenboom; G Winter
Journal:  J Mol Biol       Date:  1992-09-20       Impact factor: 5.469

7.  Expression of the cell surface antigen detected by the monoclonal antibody A7 in pancreatic carcinoma cell lines.

Authors:  E Otsuji; T Yamaguchi; N Yamaguchi; K Koyama; J Imanishi; N Yamaoka; T Takahashi
Journal:  Surg Today       Date:  1992       Impact factor: 2.549

8.  A monoclonal antibody, KM10 reactive with human gastrointestinal cancer and its application for immunotherapy.

Authors:  H Ohyanagi; H Ishida; T Ishida; N Soyama; M Yamamoto; S Okumura; Y Kano; Y Ueda; Y Saitoh
Journal:  Jpn J Cancer Res       Date:  1988-12

9.  Follow-up study of patients treated with monoclonal antibody-drug conjugate: report of 77 cases with colorectal cancer.

Authors:  T Takahashi; T Yamaguchi; K Kitamura; A Noguchi; M Honda; E Otsuji
Journal:  Jpn J Cancer Res       Date:  1993-09

10.  Enhanced tumor localization of radiolabeled Fab fragments of monoclonal antibody A7 in nude mice bearing human pancreatic carcinoma xenografts.

Authors:  E Otsuji; T Yamaguchi; N Yamaoka; M Kato; T Kotani; K Kitamura; N Yamaguchi; T Takahashi
Journal:  Jpn J Cancer Res       Date:  1993-08
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  1 in total

1.  Decreased renal accumulation of biotinylated chimeric monoclonal antibody-neocarzinostatin conjugate after administration of avidin.

Authors:  E Otsuji; T Yamaguchi; K Yamamoto; H Matsumura; H Tsuruta; Y Yata; H Nishi; K Okamoto; K Kitamura; T Takahashi
Journal:  Jpn J Cancer Res       Date:  1997-02
  1 in total

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