Literature DB >> 86291

Three-dimensional architecture of the golgi apparatus in Sertoli cells of the rat.

A Rambourg, Y Clermont, L Hermo.   

Abstract

Glutaraldehyde-fixed testes were stained "en bloc" with the Ur-Pb-Cu technique of Thiéry and Rambourg ('76) or post-fixed and stained with the osmium tetroxide-potassium ferrocyanide method of Karnovsky ('71). Thin or thick (up to 3 micron) sections were examined with the Philips (301 or 400) EM or the high voltage EM. Stereopairs were prepared with photographs of tilted specimens (+/- 7 degrees). At low magnification, in thick sections (0.5-3 micron) stained with Ur-Pb-Cu, the whole Golgi apparatus formed a single network of interconnected wavy ribbon or platelike structures extending from the juxtanuclear region toward the apex of the cell. At higher magnifications, with the two staining techniques, this Golgi network showed two distinct types of regions: the "saccular region" corresponding to the conventional stack of saccules and the "intersaccular connecting region" made up of anastomotic tubules which bridge adjacent stacks. In the saccurlar regions, there was, on the cis-face of the stack, a tight polygonal meshwork of anastomotic tubules (osmiophilic element). Underlying it there were three to seven closely apposed saccules perforated with pores of various diameters, and finally, on the trans-face, a network of tubules was usually connected to the last saccule of the stack, which seemed to peel off" from the pile. The intersaccular connecting regions showed proximal and distal zones with regard to the associated stacks. The proximal zone was made up of superimposed and parallel polygonal networks of membranous tubules which were continuous with corresponding saccules of the stack. In the distal zone they interdigitated, intertwined, anastomosed and bridged adjacent saccular regions; others turned at right angles and established connections with tubular extensions arising at various levels of the same stack. While cisternae of endoplasmic reticulum were contiguous with tubules or saccules located on the transface of the Golgi apparatus, a close association between the ER cisternae and the cis-face of the stacks was not usually observed.

Entities:  

Mesh:

Substances:

Year:  1979        PMID: 86291     DOI: 10.1002/aja.1001540402

Source DB:  PubMed          Journal:  Am J Anat        ISSN: 0002-9106


  43 in total

1.  Structure of the Golgi and distribution of reporter molecules at 20 degrees C reveals the complexity of the exit compartments.

Authors:  Mark S Ladinsky; Christine C Wu; Shane McIntosh; J Richard McIntosh; Kathryn E Howell
Journal:  Mol Biol Cell       Date:  2002-08       Impact factor: 4.138

Review 2.  The Sertoli cell: one hundred fifty years of beauty and plasticity.

Authors:  L R França; R A Hess; J M Dufour; M C Hofmann; M D Griswold
Journal:  Andrology       Date:  2016-02-04       Impact factor: 3.842

3.  Multiple Rab GTPase binding sites in GCC185 suggest a model for vesicle tethering at the trans-Golgi.

Authors:  Garret L Hayes; Frank C Brown; Alexander K Haas; Ryan M Nottingham; Francis A Barr; Suzanne R Pfeffer
Journal:  Mol Biol Cell       Date:  2008-10-22       Impact factor: 4.138

Review 4.  The many routes of Golgi-dependent trafficking.

Authors:  Gaelle Boncompain; Franck Perez
Journal:  Histochem Cell Biol       Date:  2013-07-12       Impact factor: 4.304

Review 5.  Topology-based abstraction of complex biological systems: application to the Golgi apparatus.

Authors:  Mathieu Poudret; Agnès Arnould; Jean-Paul Comet; Pascale Le Gall; Philippe Meseure; François Képès
Journal:  Theory Biosci       Date:  2008-05-06       Impact factor: 1.919

6.  Thiamine pyrophosphatase cytochemistry in rat endometrium during the oestrous cycle.

Authors:  L Staneva-Dobrovski
Journal:  Histochemistry       Date:  1994-08

7.  Golgi membrane dynamics.

Authors:  J F Presley; C Smith; K Hirschberg; C Miller; N B Cole; K J Zaal; J Lippincott-Schwartz
Journal:  Mol Biol Cell       Date:  1998-07       Impact factor: 4.138

Review 8.  Architecture of the mammalian Golgi.

Authors:  Judith Klumperman
Journal:  Cold Spring Harb Perspect Biol       Date:  2011-07-01       Impact factor: 10.005

9.  Pubertal development of intersertoli cell junctions in the testis of corriedale ram lambs.

Authors:  A Bielli; T Gastel; A Castrillejo; A Moraña; H Rodriguez-Martinez
Journal:  Acta Vet Scand       Date:  1995       Impact factor: 1.695

10.  ER-to-Golgi transport by COPII vesicles in Arabidopsis involves a ribosome-excluding scaffold that is transferred with the vesicles to the Golgi matrix.

Authors:  Byung-Ho Kang; L Andrew Staehelin
Journal:  Protoplasma       Date:  2008-09-20       Impact factor: 3.356

View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.