Literature DB >> 8629024

A U1/U4/U5 snRNP complex induced by a 2'-O-methyl-oligoribonucleotide complementary to U5 snRNA.

G Ast1, A M Weiner.   

Abstract

Nuclear messenger RNA splicing involves multiple interactions between the five spliceosomal small nuclear ribonucleoprotein particles (snRNPs) U1, U2, U4, U5, and U6 and numerous spliceosomal proteins. Here it is shown that binding of a 2'-O-methyl-oligoribonucleotide complementary to U5 small nuclear RNA (snRNA) nucleotides 68 to 88 (BU5Ae) disrupts the initial U4/U5/U6 tri-snRNP complex, enhances the U2/U6 interaction, and induces a Ul/U4/U5 snRNP complex. The Ul/U4/U5 snRNP complex interacts specifically with an RNA oligonucleotide containing the 5' splice site sequence and may therefore represent a transitional stage in the displacement of U1 from the 5' splice site by U5 snRNP.

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Year:  1996        PMID： 8629024     DOI： 10.1126/science.272.5263.881

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  8 in total

1.  Sequences upstream of the branch site are required to form helix II between U2 and U6 snRNA in a trans-splicing reaction.

Authors:  G Ast; T Pavelitz; A M Weiner
Journal:  Nucleic Acids Res       Date:  2001-04-15       Impact factor: 16.971

2.  The U1 snRNP base pairs with the 5' splice site within a penta-snRNP complex.

Authors:  Hadar Malca; Noam Shomron; Gil Ast
Journal:  Mol Cell Biol       Date:  2003-05       Impact factor: 4.272

3.  Reversible inhibition of the second step of splicing suggests a possible role of zinc in the second step of splicing.

Authors:  Noam Shomron; Hadar Malca; Ida Vig; Gil Ast
Journal:  Nucleic Acids Res       Date:  2002-10-01       Impact factor: 16.971

4.  Splicing factor hSlu7 contains a unique functional domain required to retain the protein within the nucleus.

Authors:  Noam Shomron; Mika Reznik; Gil Ast
Journal:  Mol Biol Cell       Date:  2004-06-04       Impact factor: 4.138

5.  Antisense oligonucleotide binding to U5 snRNP induces a conformational change that exposes the conserved loop of U5 snRNA.

Authors:  G Ast; A M Weiner
Journal:  Nucleic Acids Res       Date:  1997-09-01       Impact factor: 16.971

6.  Proximity of the U12 snRNA with both the 5' splice site and the branch point during early stages of spliceosome assembly.

Authors:  Mikko J Frilander; Xiaojuan Meng
Journal:  Mol Cell Biol       Date:  2005-06       Impact factor: 4.272

7.  Synthetic lethality of yeast slt mutations with U2 small nuclear RNA mutations suggests functional interactions between U2 and U5 snRNPs that are important for both steps of pre-mRNA splicing.

Authors:  D Xu; D J Field; S J Tang; A Moris; B P Bobechko; J D Friesen
Journal:  Mol Cell Biol       Date:  1998-04       Impact factor: 4.272

Review 8.  Structural dynamics of the N-terminal domain and the Switch loop of Prp8 during spliceosome assembly and activation.

Authors:  Xu Jia; Chengfu Sun
Journal:  Nucleic Acids Res       Date:  2018-05-04       Impact factor: 16.971
  8 in total

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