| Literature DB >> 8624783 |
M Sakai1, A Miyazaki, H Hakamata, Y Sato, T Matsumura, S Kobori, M Shichiri, S Horiuchi.
Abstract
The growth of murine peritoneal macrophages is induced by oxidized LDL (Ox-LDL), and lysophosphatidylcholine (lysoPC) plays an important role in its mitogenic activity. In the present++ study, Ox-LDL-induced macrophage growth was examined with human monocyte-derived macrophages. The cell growth of human macrophages was significantly induced by Ox-LDL but not by acetylated LDL (acetyl-LDL). The treatment of acetyl-LDL with phospholipase A2, however, led to a marked increase in its mitogenic activity, with a concomitant conversion of 75% of its phospholipids to lysoPC. The growth-stimulating activity became positive only when both acetyl-LDL and lysoPC were coincubated, although neither of them exhibited cell growth-promoting activity. These results suggest that Ox-LDL could stimulate the growth of human monocyte-derived macrophages, and lysoPC may play an essential role in the mitogenic activity of Ox-LDL.Entities:
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Year: 1996 PMID： 8624783 DOI： 10.1161/01.atv.16.4.600
Source DB: PubMed Journal: Arterioscler Thromb Vasc Biol ISSN： 1079-5642 Impact factor: 8.311