Literature DB >> 8624413

cDNA cloning and gene expression of ascorbate oxidase in tobacco.

N Kato1, M Esaka.   

Abstract

A cDNA clone for ascorbate oxidase (AAO) has been isolated from a cDNA library of tobacco (Nicotiana tabacum) cells. The identity of the amino acid sequence deduced from tobacco AAO cDNA to that from pumpkin AAO cDNA was 68%, which was much lower than the identity (80%) between pumpkin and cucumber AAO. AAO activity in tobacco cells was much lower than that in pumpkin cells, whereas the immunoreactive protein in tobacco cells was more abundant than that in pumpkin cells. We suppose that AAO protein in tobacco cells may be less active than that in pumpkin cells. Genomic Southern blotting suggested that AAO in tobacco was encoded by a single-copy gene. Nothern blotting revealed that mRNA of AAO was highly expressed in young and growing tissues of tobacco plant.

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Year:  1996        PMID： 8624413     DOI： 10.1007/bf00019015

Source DB:  PubMed          Journal:  Plant Mol Biol        ISSN： 0167-4412            Impact factor:   4.076


  12 in total

1.  Citrus Fruit Enzymes. I. Ascorbic Acid Oxidase in Oranges.

Authors:  H M Vines; M F Oberbacher
Journal:  Plant Physiol       Date:  1963-05       Impact factor: 8.340

2.  Marked increase in ascorbate oxidase protein in pumpkin callus by adding copper.

Authors:  M Esaka; M Uchida; H Fukui; K Kubota; K Suzuki
Journal:  Plant Physiol       Date:  1988-11       Impact factor: 8.340

3.  Primary structure of cucumber (Cucumis sativus) ascorbate oxidase deduced from cDNA sequence: homology with blue copper proteins and tissue-specific expression.

Authors:  J Ohkawa; N Okada; A Shinmyo; M Takano
Journal:  Proc Natl Acad Sci U S A       Date:  1989-02       Impact factor: 11.205

4.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

5.  Purification and properties of ascorbate oxidase from cucumber.

Authors:  T Nakamura; N Makino; Y Ogura
Journal:  J Biochem       Date:  1968-08       Impact factor: 3.387

6.  A simple and very efficient method for generating cDNA libraries.

Authors:  U Gubler; B J Hoffman
Journal:  Gene       Date:  1983-11       Impact factor: 3.688

7.  Ascorbate oxidase. Further studies on the purification of the enzyme.

Authors:  M H Lee; C R Dawson
Journal:  J Biol Chem       Date:  1973-10-10       Impact factor: 5.157

8.  Regulation of ascorbate oxidase expression in pumpkin by auxin and copper.

Authors:  M Esaka; K Fujisawa; M Goto; Y Kisu
Journal:  Plant Physiol       Date:  1992-09       Impact factor: 8.340

9.  Refined crystal structure of ascorbate oxidase at 1.9 A resolution.

Authors:  A Messerschmidt; R Ladenstein; R Huber; M Bolognesi; L Avigliano; R Petruzzelli; A Rossi; A Finazzi-Agró
Journal:  J Mol Biol       Date:  1992-03-05       Impact factor: 5.469

10.  Ascorbate electrode for determination of L-ascorbic acid in food.

Authors:  K Matsumoto; K Yamada; Y Osajima
Journal:  Anal Chem       Date:  1981-11       Impact factor: 6.986
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  8 in total

1.  Auxin metabolism in the root apical meristem.

Authors:  N M Kerk; K Jiang; L J Feldman
Journal:  Plant Physiol       Date:  2000-03       Impact factor: 8.340

2.  Melon ascorbate oxidase: cloning of a multigene family, induction during fruit development and repression by wounding.

Authors:  G Diallinas; I Pateraki; M Sanmartin; A Scossa; E Stilianou; N J Panopoulos; A K Kanellis
Journal:  Plant Mol Biol       Date:  1997-07       Impact factor: 4.076

3.  Secreted proteins of tobacco cultured BY2 cells: identification of a new member of pathogenesis-related proteins.

Authors:  Y Okushima; N Koizumi; T Kusano; H Sano
Journal:  Plant Mol Biol       Date:  2000-02       Impact factor: 4.076

4.  Generation and properties of ascorbic acid-overproducing transgenic tobacco cells expressing sense RNA for l-galactono-1,4-lactone dehydrogenase.

Authors:  Takaaki Tokunaga; Katsunori Miyahara; Kazufumi Tabata; Muneharu Esaka
Journal:  Planta       Date:  2004-11-12       Impact factor: 4.116

5.  Cloning and Functional Analysis of the Promoter of an Ascorbate Oxidase Gene from Gossypium hirsutum.

Authors:  Shan Xin; Chengcheng Tao; Hongbin Li
Journal:  PLoS One       Date:  2016-09-06       Impact factor: 3.240

6.  Cotton Ascorbate Oxidase Promotes Cell Growth in Cultured Tobacco Bright Yellow-2 Cells through Generation of Apoplast Oxidation.

Authors:  Rong Li; Shan Xin; Chengcheng Tao; Xiang Jin; Hongbin Li
Journal:  Int J Mol Sci       Date:  2017-06-23       Impact factor: 5.923

7.  Genome-Wide Identification and Expression Analysis of the Ascorbate Oxidase Gene Family in Gossypium hirsutum Reveals the Critical Role of GhAO1A in Delaying Dark-Induced Leaf Senescence.

Authors:  Ze Pan; Lihua Chen; Fei Wang; Wangyang Song; Aiping Cao; Shuangquan Xie; Xifeng Chen; Xiang Jin; Hongbin Li
Journal:  Int J Mol Sci       Date:  2019-12-06       Impact factor: 5.923

8.  Movement Protein of Cucumber Mosaic Virus Associates with Apoplastic Ascorbate Oxidase.

Authors:  Reenu Kumari; Surender Kumar; Lakhmir Singh; Vipin Hallan
Journal:  PLoS One       Date:  2016-09-26       Impact factor: 3.240
  8 in total

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