Literature DB >> 862233

A radiomicroassay for cytotoxic antibody to human spermatozoa. Quantification by tritiated actinomycin d.

J S Sung, H Shizuya, D D Black, D M Mumford.   

Abstract

A radiomicroassay for titration of spermocytotoxic antibody is described. The assay used [3H]AACTINOMYCIN D ([3H]Act D) to label damaged spermatozoa in a fashion analogous to penetration by vital dye. Optimal conditions for and some kinetics of the assay are presented. The assay is sensitive, reliable, simple to perform and uses only small amounts of serum and spermatozoa. Applied to sperm antibody positive human postvasectomy sera, the assay compared favourably in sensitivity eith vital dye microscopic observations and with parallel titration by the Isojima's immobilization tests.

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Year:  1977        PMID： 862233      PMCID： PMC1540921     

Source DB:  PubMed          Journal:  Clin Exp Immunol        ISSN： 0009-9104            Impact factor:   4.330


  9 in total

1.  THE COMPLEMENT FIXATION TEST IN VIRUS ANTIBODY STUDIES.

Authors:  A N DELAAT
Journal:  Can J Med Technol       Date:  1964-04

Review 2.  Sperm immunology, infertility, and fertility control.

Authors:  T S Li
Journal:  Obstet Gynecol       Date:  1974-10       Impact factor: 7.661

3.  Stereochemistry of actinomycin binding to DNA. II. Detailed molecular model of actinomycin-DNA complex and its implications.

Authors:  H M Sobell; S C Jain
Journal:  J Mol Biol       Date:  1972-07-14       Impact factor: 5.469

4.  The ultrastructural lesions induced by antibody and complement in rabbit spermatozoa.

Authors:  J Russo; C B Metz
Journal:  Biol Reprod       Date:  1974-04       Impact factor: 4.285

5.  Immunologic implications of vasectomy. II. Serum-mediated immunity.

Authors:  G E Brannen; D S Coffey
Journal:  Fertil Steril       Date:  1974-06       Impact factor: 7.329

6.  A simple and versatile harvesting device for processing radioactive label incorporated into and-or released from cells in microculture.

Authors:  M R Harrison; G B Thurman; G M Thomas
Journal:  J Immunol Methods       Date:  1974-01       Impact factor: 2.303

7.  Interrelation between high levels of sperm antibodies, reduced penetration of cervical mucus by spermatozoa, and sterility in men.

Authors:  B Fjällbrant
Journal:  Acta Obstet Gynecol Scand       Date:  1968       Impact factor: 3.636

8.  Antibody binding of spermatozoa.

Authors:  R W Noyes
Journal:  Fertil Steril       Date:  1969 Jan-Feb       Impact factor: 7.329

9.  Microtechnique for simultaneous determination of immobilizing and cytotoxic sperm antibodies. Methodological and clinical studies.

Authors:  S Husted; T Hjort
Journal:  Clin Exp Immunol       Date:  1975-11       Impact factor: 4.330
  9 in total
  3 in total

Review 1.  Complement in human reproduction: activation and control.

Authors:  I A Rooney; T J Oglesby; J P Atkinson
Journal:  Immunol Res       Date:  1993       Impact factor: 2.829

2.  Membrane attack complex (MAC)-mediated damage to spermatozoa: protection of the cells by the presence on their membranes of MAC inhibitory proteins.

Authors:  I A Rooney; A Davies; B P Morgan
Journal:  Immunology       Date:  1992-03       Impact factor: 7.397

3.  Lack of prostaglandin E2-mediated monocyte suppressive activity in newborn and mothers.

Authors:  A Fischer; A Durandy; S Mamas; E McCall; F Dray; C Griscelli
Journal:  Clin Exp Immunol       Date:  1982-08       Impact factor: 4.330
  3 in total

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