Literature DB >> 8621077

Introducing StuI sites improves vectors for the expression of fusion proteins with factor Xa cleavage sites.

J Görlach1, J Schmid.   

Abstract

The Arg-encoding triplet (AGG) in the recognition sequence Ile-Glu-Gly-Arg for factor Xa can be used to generate a StuI restriction site (AGGCCT) which greatly facilitates the construction of DNA fragments encoding fusion proteins. Following proteolytic cleavage with factor Xa, a protein with the desired N terminus can be obtained.

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Year:  1996        PMID： 8621077     DOI： 10.1016/0378-1119(95)00825-x

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  4 in total

1.  Molecular characterization of tomato 3-dehydroquinate dehydratase-shikimate:NADP oxidoreductase.

Authors:  M Bischoff; A Schaller; F Bieri; F Kessler; N Amrhein; J Schmid
Journal:  Plant Physiol       Date:  2001-04       Impact factor: 8.340

2.  Cinnamate:CoA ligase initiates the biosynthesis of a benzoate-derived xanthone phytoalexin in Hypericum calycinum cell cultures.

Authors:  Mariam M Gaid; Debabrata Sircar; Andreas Müller; Till Beuerle; Benye Liu; Ludger Ernst; Robert Hänsch; Ludger Beerhues
Journal:  Plant Physiol       Date:  2012-09-19       Impact factor: 8.340

3.  Maize phenylalanine ammonia-lyase has tyrosine ammonia-lyase activity.

Authors:  J Rösler; F Krekel; N Amrhein; J Schmid
Journal:  Plant Physiol       Date:  1997-01       Impact factor: 8.340

4.  Benzophenone Synthase and Chalcone Synthase Accumulate in the Mesophyll of Hypericum perforatum Leaves at Different Developmental Stages.

Authors:  Asma K Belkheir; Mariam Gaid; Benye Liu; Robert Hänsch; Ludger Beerhues
Journal:  Front Plant Sci       Date:  2016-06-29       Impact factor: 5.753
  4 in total

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