B L Roeder1, B Schaalje, E J Kelly, F D Clark. 1. Department of Animal Science, College of Biology and Agriculture, Brigham Young University, Provo, UT 84602, USA.
Abstract
OBJECTIVE: To determine an accurate rapid method for determination of blood glucose concentration in cattle under field conditions. DESIGN: Prospective, randomized, controlled trial. ANIMALS: 62 clinically normal Holstein cattle: 34 cows and 28 calves. PROCEDURE: Glucose concentrations in venous blood samples were measured in duplicate using a rapid, dry-slide chemistry technique for determination of blood glucose concentration and a laboratory-based method for determination of plasma glucose concentration. Analyses of variance were used to determine whether the relationship between results of the 2 methods was affected by the status of the animals (cows vs calves) or the PCV of the blood samples. Simple linear regression was performed to determine the correlation between the 2 methods and the slope, intercept, and residual error variance of the relationship between the methods. RESULTS: There as a significant linear relationship between the 2 methods throughout the range of glucose concentrations. Mean difference between results of the 2 methods (results for laboratory-based method - results for rapid method) was 12.95 mg/dl (SD, 7.20 mg/dl). The PCV did not affect the relationship, and there was no difference between results of the 2 methods for cows versus calves. Correlation between means of the duplicative values determined by use of the 2 methods was high (r = 0.9462). CLINICAL IMPLICATIONS: The good correlation between the 2 procedures and the comparable precision estimates (coefficient of variation, 7.17% for laboratory-based method; coefficient of variation, 10.11% for rapid methods) indicates that using the rapid method to measure blood glucose concentration is valid in cows and calves.
OBJECTIVE: To determine an accurate rapid method for determination of blood glucose concentration in cattle under field conditions. DESIGN: Prospective, randomized, controlled trial. ANIMALS: 62 clinically normal Holstein cattle: 34 cows and 28 calves. PROCEDURE: Glucose concentrations in venous blood samples were measured in duplicate using a rapid, dry-slide chemistry technique for determination of blood glucose concentration and a laboratory-based method for determination of plasma glucose concentration. Analyses of variance were used to determine whether the relationship between results of the 2 methods was affected by the status of the animals (cows vs calves) or the PCV of the blood samples. Simple linear regression was performed to determine the correlation between the 2 methods and the slope, intercept, and residual error variance of the relationship between the methods. RESULTS: There as a significant linear relationship between the 2 methods throughout the range of glucose concentrations. Mean difference between results of the 2 methods (results for laboratory-based method - results for rapid method) was 12.95 mg/dl (SD, 7.20 mg/dl). The PCV did not affect the relationship, and there was no difference between results of the 2 methods for cows versus calves. Correlation between means of the duplicative values determined by use of the 2 methods was high (r = 0.9462). CLINICAL IMPLICATIONS: The good correlation between the 2 procedures and the comparable precision estimates (coefficient of variation, 7.17% for laboratory-based method; coefficient of variation, 10.11% for rapid methods) indicates that using the rapid method to measure blood glucose concentration is valid in cows and calves.
Authors: B Mair; M Drillich; D Klein-Jöbstl; P Kanz; S Borchardt; L Meyer; I Schwendenwein; M Iwersen Journal: BMC Vet Res Date: 2016-02-24 Impact factor: 2.741