BACKGROUND: Tumor suppressor genes are important in the development of head and neck cancer. Using microsatellite markers that map close to the region 3p24-pter, we determined the frequency of allele loss close to this site with a view to narrowing the search for a putative tumor suppressor gene involved in the development of squamous cell carcinoma of the head and neck, which may facilitate future positional cloning techniques. DESIGN: Laboratory-based project with tumor and normal specimens subjected to molecular genetic analysis. Tumor-normal tissue DNA pairs were analyzed for allelic imbalance and microsatellite instability on chromosome 3p in the region 3p24-pter by the polymerase chain reaction and microsatellite markers D3S1304, D3S656, D3S1252, D3S1293, THRB, and D3S1266. SETTING: Molecular genetics and oncology research laboratory. PATIENTS: Paired tumor-normal DNA samples were obtained from 46 patients with tumors of the head and neck. MAIN OUTCOME MEASURES: Detection of loss of heterozygosity and microsatellite instability on chromosome 3 in the region 3p24-p25.1. RESULTS: We found loss of heterozygosity with at least one marker in 48% of informative cases and loss of heterozygosity or microsatellite instability in 57% of informative cases. The minimal region of loss was found in the region bounded by D3S656 and D31293. CONCLUSION: A putative tumor suppressor gene in head and neck cancer lies between D3S656 and D3S1293 in the 3p25.1 region.
BACKGROUND:Tumor suppressor genes are important in the development of head and neck cancer. Using microsatellite markers that map close to the region 3p24-pter, we determined the frequency of allele loss close to this site with a view to narrowing the search for a putative tumor suppressor gene involved in the development of squamous cell carcinoma of the head and neck, which may facilitate future positional cloning techniques. DESIGN: Laboratory-based project with tumor and normal specimens subjected to molecular genetic analysis. Tumor-normal tissue DNA pairs were analyzed for allelic imbalance and microsatellite instability on chromosome 3p in the region 3p24-pter by the polymerase chain reaction and microsatellite markers D3S1304, D3S656, D3S1252, D3S1293, THRB, and D3S1266. SETTING: Molecular genetics and oncology research laboratory. PATIENTS: Paired tumor-normal DNA samples were obtained from 46 patients with tumors of the head and neck. MAIN OUTCOME MEASURES: Detection of loss of heterozygosity and microsatellite instability on chromosome 3 in the region 3p24-p25.1. RESULTS: We found loss of heterozygosity with at least one marker in 48% of informative cases and loss of heterozygosity or microsatellite instability in 57% of informative cases. The minimal region of loss was found in the region bounded by D3S656 and D31293. CONCLUSION: A putative tumor suppressor gene in head and neck cancer lies between D3S656 and D3S1293 in the 3p25.1 region.
Authors: Aleksandr G Kondratov; Sergiy M Kvasha; Liubov A Stoliar; Alina M Romanenko; Yury M Zgonnyk; Vasily V Gordiyuk; Elena V Kashuba; Alla V Rynditch; Eugene R Zabarovsky; Vladimir I Kashuba Journal: PLoS One Date: 2012-10-08 Impact factor: 3.240
Authors: Vladimir Kashuba; Alexey A Dmitriev; George S Krasnov; Tatiana Pavlova; Ilya Ignatjev; Vasily V Gordiyuk; Anna V Gerashchenko; Eleonora A Braga; Surya P Yenamandra; Michael Lerman; Vera N Senchenko; Eugene Zabarovsky Journal: Int J Mol Sci Date: 2012-10-18 Impact factor: 5.923