Literature DB >> 8615641

Identification and tissue-specific expression of a NADH-dependent activity of dihydropyrimidine dehydrogenase in man.

A B Van Kuilenburg1, H Van Lenthe, A H Van Gennip.   

Abstract

Homogenates of human liver and human fibroblasts were able to convert thymine into dihydrothymine in the presence of NADH whereas almost no NADH-dependent activity could be detected in human lymphocytes. The different tissue distribution of the NADH-dependent activity suggests that different types of human dihydropyrimidine dehydrogenase exist. Both types of human liver dihydropyrimidine dehydrogenase showed a comparable affinity towards thymine, NADH and NADPH. Only a ten-fold lower Vmax value was observed for the NADH-dependent enzyme. During partial purification of the NADPH-dependent enzyme, on a 2', 5' - ADP Sepharose 4B column, the NADH-dependent activity was completely lost. Neither type of activity was retained on a 5' - AMP Sepharose 4B column.

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Year:  1996        PMID： 8615641

Source DB:  PubMed          Journal:  Anticancer Res        ISSN： 0250-7005            Impact factor:   2.480


  10 in total

1.  Dihydropyrimidinase deficiency and congenital microvillous atrophy: coincidence or genetic relation?

Authors:  B Assmann; G F Hoffmann; L Wagner; C Bräutigam; H W Seyberth; M Duran; A B Van Kuilenburg; R Wevers; A H Van Gennip
Journal:  J Inherit Metab Dis       Date:  1997-09       Impact factor: 4.982

2.  Dihydropyrimidine dehydrogenase deficiency: a novel mutation and expression of missense mutations in E. coli.

Authors:  P Vreken; A B van Kuilenburg; R Meinsma; F A Beemer; M Duran; A H van Gennip
Journal:  J Inherit Metab Dis       Date:  1998-06       Impact factor: 4.982

3.  Severe 5-fluorouracil toxicity caused by reduced dihydropyrimidine dehydrogenase activity due to heterozygosity for a G-->A point mutation.

Authors:  A B van Kuilenburg; P Vreken; L V Beex; R A De Abreu; A H van Gennip
Journal:  J Inherit Metab Dis       Date:  1998-06       Impact factor: 4.982

4.  Clinical variability in three Danish patients with dihydropyrimidine dehydrogenase deficiency all homozygous for the same mutation.

Authors:  E Christensen; I Cezanne; S Kjaergaard; H Hørlyk; V Faurholt Pedersen; P Vreken; A B van Kuilenburg; A H Van Gennip
Journal:  J Inherit Metab Dis       Date:  1998-06       Impact factor: 4.982

5.  The activity of dihydropyrimidine dehydrogenase in human blood cells.

Authors:  A B Van Kuilenburg; M J Blom; H Van Lenthe; E Mul; A H Van Gennip
Journal:  J Inherit Metab Dis       Date:  1997-07       Impact factor: 4.982

6.  Dihydropyrimidinase deficiency: confirmation of the enzyme defect in dihydropyrimidinuria.

Authors:  A H van Gennip; R A de Abreu; H van Lenthe; J Bakkeren; J Rotteveel; P Vreken; A B van Kuilenburg
Journal:  J Inherit Metab Dis       Date:  1997-07       Impact factor: 4.982

7.  Identification of novel point mutations in the dihydropyrimidine dehydrogenase gene.

Authors:  P Vreken; A B Van Kuilenburg; R Meinsma; A H van Gennip
Journal:  J Inherit Metab Dis       Date:  1997-07       Impact factor: 4.982

Review 8.  Inborn errors of pyrimidine degradation: clinical, biochemical and molecular aspects.

Authors:  A H van Gennip; N G Abeling; P Vreken; A B van Kuilenburg
Journal:  J Inherit Metab Dis       Date:  1997-06       Impact factor: 4.982

9.  A point mutation in an invariant splice donor site leads to exon skipping in two unrelated Dutch patients with dihydropyrimidine dehydrogenase deficiency.

Authors:  P Vreken; A B Van Kuilenburg; R Meinsma; G P Smit; H D Bakker; R A De Abreu; A H van Gennip
Journal:  J Inherit Metab Dis       Date:  1996       Impact factor: 4.982

10.  Profound variation in dihydropyrimidine dehydrogenase activity in human blood cells: major implications for the detection of partly deficient patients.

Authors:  A B Van Kuilenburg; H van Lenthe; M J Blom; E P Mul; A H Van Gennip
Journal:  Br J Cancer       Date:  1999-02       Impact factor: 7.640
  10 in total

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