| Literature DB >> 8612755 |
I D Prijambada1, T Yomo, F Tanaka, T Kawama, K Yamamoto, A Hasegawa, Y Shima, S Negoro, I Urabe.
Abstract
A library of artificial random proteins of 141 amino acid residues of which 95 are random and which includes the 20 kinds of amino acids was prepared. Out of the 25 identified random proteins, 5 were soluble in the cell lysate, indicating that about 20% of the random proteins expressed in Escherichia coli are expected to be soluble. The soluble random proteins RP3-42 and RP3-45 and insoluble RP3-70 were purified. The solubility of the purified form is the same as that in the cell lysate.Entities:
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Year: 1996 PMID： 8612755 DOI： 10.1016/0014-5793(96)00123-8
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124