| Literature DB >> 8611948 |
R Navajas1, C Imaz, D Carreras, M García, M Pérez, C Rodríguez, A F Rodríguez, R Cortés.
Abstract
This paper describes an alternative HPLC method for the determination of testosterone and epitestosterone, which is simple, rapid, selective, sensitive and reproducible. Samples were prepared using a previous enzymatic hydrolysis with liquid-liquid extraction. The determination was carried out on a Hypersil BDS-C18 reversed-phase column with gradient elution and UV absorbance detection (240 nm). The limits of quantification (signal-to-noise ratio = 6) were 20 ng/ml for testosterone and 30 ng/ml for epitestosterone.Entities:
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Year: 1995 PMID： 8611948 DOI： 10.1016/0378-4347(95)00260-x
Source DB: PubMed Journal: J Chromatogr B Biomed Appl ISSN： 1572-6495