Literature DB >> 8610152

Formation of chimeric DNA primer extension products by template switching onto an annealed downstream oligonucleotide.

R Patel1, M Lin, M Laney, N Kurn, S Rose, E F Ullman.   

Abstract

Oligodeoxynucleotide sequences are described that anneal to a template downstream of a priming site. During polymerase-catalyzed extension of the primer, the extending primer shifts from the original template to a segment of the annealed oligonucleotide that acts as an alternative template. The resulting chimeric extended primer has one segment that is complementary to the template and a second segment that is complementary to the oligonucleotide. The influence of the sequence elements of the oligonucleotide and the reaction conditions on template switching have been explored. The sequence requirements for template switching are compared to those for transposon excision.

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Year:  1996        PMID： 8610152      PMCID： PMC39744          DOI： 10.1073/pnas.93.7.2969

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  12 in total

1.  ENZYMIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XVII. SOME UNUSUAL PHYSICAL PROPERTIES OF THE PRODUCT PRIMED BY NATIVE DNA TEMPLATES.

Authors:  C L SCHILDKRAUT; C C RICHARDSON; A KORNBERG
Journal:  J Mol Biol       Date:  1964-07       Impact factor: 5.469

2.  Sequence-independent, single-primer amplification (SISPA) of complex DNA populations.

Authors:  G R Reyes; J P Kim
Journal:  Mol Cell Probes       Date:  1991-12       Impact factor: 2.365

3.  Elementary steps of base recognition and helix-coil transitions in nucleic acids.

Authors:  D Pörschke
Journal:  Mol Biol Biochem Biophys       Date:  1977

4.  The nucleotide sequence of the Escherichia coli K12 dnaJ+ gene. A gene that encodes a heat shock protein.

Authors:  J C Bardwell; K Tilly; E Craig; J King; M Zylicz; C Georgopoulos
Journal:  J Biol Chem       Date:  1986-02-05       Impact factor: 5.157

5.  Genetic studies of the lac repressor. VII. On the molecular nature of spontaneous hotspots in the lacI gene of Escherichia coli.

Authors:  P J Farabaugh; U Schmeissner; M Hofer; J H Miller
Journal:  J Mol Biol       Date:  1978-12-25       Impact factor: 5.469

6.  Strand displacement during deoxyribonucleic acid synthesis at single strand breaks.

Authors:  Y Masamune; C C Richardson
Journal:  J Biol Chem       Date:  1971-04-25       Impact factor: 5.157

7.  Excision of transposon Tn5 is dependent on the inverted repeats but not on the transposase function of Tn5.

Authors:  C Egner; D E Berg
Journal:  Proc Natl Acad Sci U S A       Date:  1981-01       Impact factor: 11.205

8.  Requirements for the catalysis of strand transfer synthesis by retroviral DNA polymerases.

Authors:  R G Buiser; J J DeStefano; L M Mallaber; P J Fay; R A Bambara
Journal:  J Biol Chem       Date:  1991-07-15       Impact factor: 5.157

9.  Requirements for strand transfer between internal regions of heteropolymer templates by human immunodeficiency virus reverse transcriptase.

Authors:  J J DeStefano; L M Mallaber; L Rodriguez-Rodriguez; P J Fay; R A Bambara
Journal:  J Virol       Date:  1992-11       Impact factor: 5.103

10.  Transposon Tn5 excision in yeast: influence of DNA polymerases alpha, delta, and epsilon and repair genes.

Authors:  D A Gordenin; A L Malkova; A Peterzen; V N Kulikov; Y I Pavlov; E Perkins; M A Resnick
Journal:  Proc Natl Acad Sci U S A       Date:  1992-05-01       Impact factor: 11.205
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  9 in total

1.  A high-throughput Arabidopsis reverse genetics system.

Authors:  Allen Sessions; Ellen Burke; Gernot Presting; George Aux; John McElver; David Patton; Bob Dietrich; Patrick Ho; Johana Bacwaden; Cynthia Ko; Joseph D Clarke; David Cotton; David Bullis; Jennifer Snell; Trini Miguel; Don Hutchison; Bill Kimmerly; Theresa Mitzel; Fumiaki Katagiri; Jane Glazebrook; Marc Law; Stephen A Goff
Journal:  Plant Cell       Date:  2002-12       Impact factor: 11.277

2.  Reevaluation and reduction of a PCR bias caused by reannealing of templates.

Authors:  Shinya Kurata; Takahiro Kanagawa; Yukio Magariyama; Kyoko Takatsu; Kazutaka Yamada; Toyokazu Yokomaku; Yoichi Kamagata
Journal:  Appl Environ Microbiol       Date:  2004-12       Impact factor: 4.792

3.  Rationalizing molecular analysis of field-collected roots for assessing diversity of arbuscular mycorrhizal fungi: to pool, or not to pool, that is the question.

Authors:  C Renker; K Weißhuhn; H Kellner; F Buscot
Journal:  Mycorrhiza       Date:  2006-09-16       Impact factor: 3.387

4.  Formation of template-switching artifacts by linear amplification.

Authors:  Dhrubajyoti Chakravarti; Paula C Mailander
Journal:  J Biomol Tech       Date:  2008-07

5.  Rolling circle replication requires single-stranded DNA binding protein to avoid termination and production of double-stranded DNA.

Authors:  Cosimo Ducani; Giulio Bernardinelli; Björn Högberg
Journal:  Nucleic Acids Res       Date:  2014-08-12       Impact factor: 16.971

6.  Unbiased quantification of immunoglobulin diversity at the DNA level with VDJ-seq.

Authors:  Peter Chovanec; Daniel J Bolland; Louise S Matheson; Andrew L Wood; Felix Krueger; Simon Andrews; Anne E Corcoran
Journal:  Nat Protoc       Date:  2018-05-03       Impact factor: 13.491

7.  A Survey of Virus Recombination Uncovers Canonical Features of Artificial Chimeras Generated During Deep Sequencing Library Preparation.

Authors:  Jean Peccoud; Sébastian Lequime; Isabelle Moltini-Conclois; Isabelle Giraud; Louis Lambrechts; Clément Gilbert
Journal:  G3 (Bethesda)       Date:  2018-03-28       Impact factor: 3.154

8.  Long-read metagenomic exploration of extrachromosomal mobile genetic elements in the human gut.

Authors:  Yoshihiko Suzuki; Suguru Nishijima; Yoshikazu Furuta; Jun Yoshimura; Wataru Suda; Kenshiro Oshima; Masahira Hattori; Shinichi Morishita
Journal:  Microbiome       Date:  2019-08-27       Impact factor: 14.650

9.  Correlation of the 4977 bp mitochondrial DNA deletion with human sperm dysfunction.

Authors:  Fotini Ieremiadou; George C Rodakis
Journal:  BMC Res Notes       Date:  2009-02-04
  9 in total

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