| Literature DB >> 8606121 |
G Lépine1, R P Ellen, A Progulske-Fox.
Abstract
Targeted insertional mutagenesis was used to construct hagA, hagB, and hagC hemagglutinin mutants of Porphyromonas gingivalis. pJRD215-derived plasmids containing tetA(Q)2 and portions of the targeted genes were conjugated into P. gingivalis. Interruption of the three loci was confirmed by Southern hybridization, sequencing, reverse transcription-PCR, and microtiter hemagglutination assays. No significant differences in hydrophobicity or coadherence to Actinomyces viscosus were detected between the mutants and the wild-type strain.Entities:
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Year: 1996 PMID: 8606121 PMCID: PMC173946 DOI: 10.1128/iai.64.4.1467-1472.1996
Source DB: PubMed Journal: Infect Immun ISSN: 0019-9567 Impact factor: 3.441