Literature DB >> 8606121

Construction and preliminary characterization of three hemagglutinin mutants of Porphyromonas gingivalis.

G Lépine1, R P Ellen, A Progulske-Fox.   

Abstract

Targeted insertional mutagenesis was used to construct hagA, hagB, and hagC hemagglutinin mutants of Porphyromonas gingivalis. pJRD215-derived plasmids containing tetA(Q)2 and portions of the targeted genes were conjugated into P. gingivalis. Interruption of the three loci was confirmed by Southern hybridization, sequencing, reverse transcription-PCR, and microtiter hemagglutination assays. No significant differences in hydrophobicity or coadherence to Actinomyces viscosus were detected between the mutants and the wild-type strain.

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Year:  1996        PMID: 8606121      PMCID: PMC173946          DOI: 10.1128/iai.64.4.1467-1472.1996

Source DB:  PubMed          Journal:  Infect Immun        ISSN: 0019-9567            Impact factor:   3.441


  23 in total

1.  Vectors with restriction site banks. V. pJRD215, a wide-host-range cosmid vector with multiple cloning sites.

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Journal:  Gene       Date:  1987       Impact factor: 3.688

Review 2.  Surface recognition among oral bacteria: multigeneric coaggregations and their mediators.

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Journal:  Crit Rev Microbiol       Date:  1989       Impact factor: 7.624

3.  Attachment of Bacteroides melaninogenicus subsp. asaccharolyticus to oral surfaces and its possible role in colonization of the mouth and of periodontal pockets.

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Journal:  Infect Immun       Date:  1978-01       Impact factor: 3.441

Review 4.  Biology of asaccharolytic black-pigmented Bacteroides species.

Authors:  D Mayrand; S C Holt
Journal:  Microbiol Rev       Date:  1988-03

5.  Bacteroides gingivalis and Bacteroides intermedius recognize different sites on human fibrinogen.

Authors:  M S Lantz; R D Allen; P Bounelis; L M Switalski; M Hook
Journal:  J Bacteriol       Date:  1990-02       Impact factor: 3.490

6.  Effect of crevicular fluid and lysosomal enzymes on the adherence of streptococci and bacteroides to hydroxyapatite.

Authors:  G Cimasoni; M Song; B C McBride
Journal:  Infect Immun       Date:  1987-06       Impact factor: 3.441

7.  Hemagglutinin, urease, and hemolysin production by Proteus mirabilis from clinical sources.

Authors:  H L Mobley; G R Chippendale
Journal:  J Infect Dis       Date:  1990-03       Impact factor: 5.226

8.  Incidence of hemagglutination activity among pathogenic and non-pathogenic Bacteroides fragilis strains and role of capsule and pili in HA and adherence.

Authors:  C Pruzzo; C A Guzmán; B Dainelli
Journal:  FEMS Microbiol Lett       Date:  1989-05       Impact factor: 2.742

9.  The cloning, expression and sequence analysis of a second Porphyromonas gingivalis gene that codes for a protein involved in hemagglutination.

Authors:  A Progulske-Fox; S Tumwasorn; G Lépine; J Whitlock; D Savett; J J Ferretti; J A Banas
Journal:  Oral Microbiol Immunol       Date:  1995-10

10.  Studies on the virulence properties and metabolism of pleiotropic mutants of Porphyromonas gingivalis (Bacteroides gingivalis) W50.

Authors:  H N Shah; S V Seddon; S E Gharbia
Journal:  Oral Microbiol Immunol       Date:  1989-03
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  17 in total

1.  Localization of HArep-containing genes on the chromosome of Porphyromonas gingivalis W83.

Authors:  J P Lewis; F L Macrina
Journal:  Infect Immun       Date:  1999-05       Impact factor: 3.441

2.  The K1 serotype capsular polysaccharide of Porphyromonas gingivalis elicits chemokine production from murine macrophages that facilitates cell migration.

Authors:  Gabriela d'Empaire; Michael T Baer; Frank C Gibson
Journal:  Infect Immun       Date:  2006-08-28       Impact factor: 3.441

3.  TLR4 signaling via MyD88 and TRIF differentially shape the CD4+ T cell response to Porphyromonas gingivalis hemagglutinin B.

Authors:  Dalia E Gaddis; Suzanne M Michalek; Jannet Katz
Journal:  J Immunol       Date:  2011-04-15       Impact factor: 5.422

4.  Adsorption of components of the plasma kinin-forming system on the surface of Porphyromonas gingivalis involves gingipains as the major docking platforms.

Authors:  Maria Rapala-Kozik; Grazyna Bras; Barbara Chruscicka; Justyna Karkowska-Kuleta; Aneta Sroka; Heiko Herwald; Ky-Anh Nguyen; Sigrun Eick; Jan Potempa; Andrzej Kozik
Journal:  Infect Immun       Date:  2010-11-22       Impact factor: 3.441

5.  Both the unique and repeat regions of the Porphyromonas gingivalis hemagglutin A are involved in adhesion and invasion of host cells.

Authors:  Myriam Bélanger; Emil Kozarov; Hong Song; Joan Whitlock; Ann Progulske-Fox
Journal:  Anaerobe       Date:  2011-11-11       Impact factor: 3.331

6.  Identification and testing of Porphyromonas gingivalis virulence genes with a pPGIVET system.

Authors:  Yi Wu; Seok-Woo Lee; Jeffrey D Hillman; Ann Progulske-Fox
Journal:  Infect Immun       Date:  2002-02       Impact factor: 3.441

7.  Porphyromonas gingivalis minor fimbriae are required for cell-cell interactions.

Authors:  Xinghua Lin; Jie Wu; Hua Xie
Journal:  Infect Immun       Date:  2006-10       Impact factor: 3.441

8.  Role of fimbriae in Porphyromonas gingivalis invasion of gingival epithelial cells.

Authors:  A Weinberg; C M Belton; Y Park; R J Lamont
Journal:  Infect Immun       Date:  1997-01       Impact factor: 3.441

9.  Hemagglutinin B is involved in the adherence of Porphyromonas gingivalis to human coronary artery endothelial cells.

Authors:  Hong Song; Myriam Bélanger; Joan Whitlock; Emil Kozarov; Ann Progulske-Fox
Journal:  Infect Immun       Date:  2005-11       Impact factor: 3.441

10.  Mechanisms of monophosphoryl lipid A augmentation of host responses to recombinant HagB from Porphyromonas gingivalis.

Authors:  Qiu-Bo Yang; Michael Martin; Suzanne M Michalek; Jannet Katz
Journal:  Infect Immun       Date:  2002-07       Impact factor: 3.441

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