Literature DB >> 8601707

Comparison of protocols for depleting anti-E. coli antibody in immunoblotting of recombinant antigens.

G Covini1, E K Chan, M Colombo, E M Tan, E M Tan.   

Abstract

A common problem in immunoblotting for the detection of specific antibodies to recombinant proteins synthesized in E. coli is the presence of contaminating antibodies to E. coli proteins. Four protocols for the efficient depletion of anti-E. coli antibodies from human sera are provided and their uses are discussed.

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Year:  1996        PMID: 8601707     DOI: 10.1016/0022-1759(95)00288-x

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN: 0022-1759            Impact factor:   2.303


  2 in total

1.  Exploration of novel cellular and serological antigen biomarkers in the ORFeome of Mycobacterium tuberculosis.

Authors:  Liguo Liu; Wei-jia Zhang; Jianhua Zheng; Hua Fu; Qi Chen; Zongde Zhang; Xinchun Chen; Boping Zhou; Lu Feng; Haiying Liu; Qi Jin
Journal:  Mol Cell Proteomics       Date:  2014-01-21       Impact factor: 5.911

2.  A novel cytoplasmic protein with RNA-binding motifs is an autoantigen in human hepatocellular carcinoma.

Authors:  J Y Zhang; E K Chan; X X Peng; E M Tan
Journal:  J Exp Med       Date:  1999-04-05       Impact factor: 14.307

  2 in total

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