Literature DB >> 8601443

Analysis of the catalytic site of the actin ADP-ribosylating Clostridium perfringens iota toxin.

J van Damme1, M Jung, F Hofmann, I Just, J Vandekerckhove, K Aktories.   

Abstract

The enzyme component of actin ADP-ribosylating Clostridium perfringens iota toxin was affinity labelled by UV irradiation in the presence of [carbonyl-14C]NAD. A peptide containing the radiolabel was generated by CNBr cleavage and subsequent proteolysis with trypsin. Its amino acid sequence is Gly-Ser-Pro-Gly-Ala-Tyr-Leu-Ser-Ala-Ile-Pro-Gly-Tyr-Ala-Gly-X-Tyr-Glu-Va l-Leu-Leu-Asn-His-Gly-Ser-Lys corresponding with the region Gly-363 through Lys-388 in the C. perfringens iota toxin. Mass spectrometric data as well as results of the PTH-amino acid analysis are in line with a modification of a glutamic acid side chain located at position 378. Therefore, in addition to Glu-380, as could be concluded by analogy with other ADP-ribosyltransferases, Glu-378 may play a pivotal role in the active site of C. perfringens iota toxin.

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Year:  1996        PMID： 8601443     DOI： 10.1016/0014-5793(96)00052-x

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  17 in total

1.  Clostridium perfringens iota-toxin: mapping of receptor binding and Ia docking domains on Ib.

Authors:  J C Marvaud; T Smith; M L Hale; M R Popoff; L A Smith; B G Stiles
Journal:  Infect Immun       Date:  2001-04       Impact factor: 3.441

2.  Characterization of the enzymatic component of Clostridium perfringens iota-toxin.

Authors:  M Nagahama; Y Sakaguchi; K Kobayashi; S Ochi; J Sakurai
Journal:  J Bacteriol       Date:  2000-04       Impact factor: 3.490

3.  Arginine ADP-ribosylation mechanism based on structural snapshots of iota-toxin and actin complex.

Authors:  Toshiharu Tsurumura; Yayoi Tsumori; Hao Qiu; Masataka Oda; Jun Sakurai; Masahiro Nagahama; Hideaki Tsuge
Journal:  Proc Natl Acad Sci U S A       Date:  2013-02-04       Impact factor: 11.205

4.  Photoaffinity labelling of human poly(ADP-ribose) polymerase catalytic domain.

Authors:  H Kim; M K Jacobson; V Rolli; J Ménissier-de Murcia; J Reinbolt; F Simonin; A Ruf; G Schulz; G de Murcia
Journal:  Biochem J       Date:  1997-03-01       Impact factor: 3.857

5.  Clostridium perfringens iota toxin: binding studies and characterization of cell surface receptor by fluorescence-activated cytometry.

Authors:  B G Stiles; M L Hale; J C Marvaud; M R Popoff
Journal:  Infect Immun       Date:  2000-06       Impact factor: 3.441

6.  Production of a complete binary toxin (actin-specific ADP-ribosyltransferase) by Clostridium difficile CD196.

Authors:  S Perelle; M Gibert; P Bourlioux; G Corthier; M R Popoff
Journal:  Infect Immun       Date:  1997-04       Impact factor: 3.441

7.  ModA and ModB, two ADP-ribosyltransferases encoded by bacteriophage T4: catalytic properties and mutation analysis.

Authors:  Bernd Tiemann; Reinhard Depping; Egle Gineikiene; Laura Kaliniene; Rimas Nivinskas; Wolfgang Rüger
Journal:  J Bacteriol       Date:  2004-11       Impact factor: 3.490

8.  Clostridium perfringens type E animal enteritis isolates with highly conserved, silent enterotoxin gene sequences.

Authors:  S J Billington; E U Wieckowski; M R Sarker; D Bueschel; J G Songer; B A McClane
Journal:  Infect Immun       Date:  1998-09       Impact factor: 3.441

Review 9.  Binary bacterial toxins: biochemistry, biology, and applications of common Clostridium and Bacillus proteins.

Authors:  Holger Barth; Klaus Aktories; Michel R Popoff; Bradley G Stiles
Journal:  Microbiol Mol Biol Rev       Date:  2004-09       Impact factor: 11.056

10.  Structural basis for substrate recognition in the enzymatic component of ADP-ribosyltransferase toxin CDTa from Clostridium difficile.

Authors:  Amit Sundriyal; April K Roberts; Clifford C Shone; K Ravi Acharya
Journal:  J Biol Chem       Date:  2009-08-19       Impact factor: 5.157
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