S Aliakbar1, P R Brown. 1. Molecular Biology and Biophysics Section, Division of Biomedical Sciences, King's College London, Strand, UK.
Abstract
OBJECTIVE: The aim of the present study was to determine the erythrocyte CAI and CAII concentrations in fetal blood over a wide gestational range, and compare levels to those found in neonates and adults. METHODS: Human erythrocyte CAI and CAII concentrations were measured in fetal (n=38), neonatal (n=10) and adult (n=30) blood, using enzyme-linked immunosorbent assays (ELISA). For the measurement of CAII, a new ELISA method was developed. RESULTS AND CONCLUSIONS: The ELISA method was found to be simple, sensitive, economical, and precise. The normal mean levels of erythrocyte CAI and CAII in adults with standard deviation were 13.68 +/- 2.79, and 1.59 +/- 0.21 mg/g Hb, respectively. The corresponding values in cord blood obtained at delivery at 38-40 weeks gestation were 1.20 +/- 0.68 and 0.46 +/- 0.13 mg/g Hb. The mean CAI/CAII ratio in adults was 8.8 and, in newborns, it was 2.5. The normal mean fetal erythrocyte CAI concentration increased significantly with gestation from 39 microgram/g Hb at 20 weeks to 380 microgram/g Hb at 38 weeks of gestation. Similarly, the CAII concentration increased from 53 microgram/g Hb at 20 weeks to 120 microgram/g Hb at 38 weeks of gestation. The CAI to CAII ratio also increased with gestation from 0.9 at 20 weeks to 2.5 at 38 weeks.
OBJECTIVE: The aim of the present study was to determine the erythrocyte CAI and CAII concentrations in fetal blood over a wide gestational range, and compare levels to those found in neonates and adults. METHODS:Human erythrocyte CAI and CAII concentrations were measured in fetal (n=38), neonatal (n=10) and adult (n=30) blood, using enzyme-linked immunosorbent assays (ELISA). For the measurement of CAII, a new ELISA method was developed. RESULTS AND CONCLUSIONS: The ELISA method was found to be simple, sensitive, economical, and precise. The normal mean levels of erythrocyte CAI and CAII in adults with standard deviation were 13.68 +/- 2.79, and 1.59 +/- 0.21 mg/g Hb, respectively. The corresponding values in cord blood obtained at delivery at 38-40 weeks gestation were 1.20 +/- 0.68 and 0.46 +/- 0.13 mg/g Hb. The mean CAI/CAII ratio in adults was 8.8 and, in newborns, it was 2.5. The normal mean fetal erythrocyte CAI concentration increased significantly with gestation from 39 microgram/g Hb at 20 weeks to 380 microgram/g Hb at 38 weeks of gestation. Similarly, the CAII concentration increased from 53 microgram/g Hb at 20 weeks to 120 microgram/g Hb at 38 weeks of gestation. The CAI to CAII ratio also increased with gestation from 0.9 at 20 weeks to 2.5 at 38 weeks.
Authors: Sarah Horvath; Patricia Tsao; Zhen-Yu Huang; Ling Zhao; Yangzhu Du; Mary D Sammel; Eline T Luning Prak; Courtney A Schreiber Journal: Contraception Date: 2020-03-03 Impact factor: 3.375