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Literature DB >> 8597659

A soluble protein is imported into Euglena chloroplasts as a membrane-bound precursor.

Abstract

The Euglena precursor to the small subunit of ribulose-15-bisphosphate carboxylase/oxygenase (pSSU) is a polyprotein. To determine the transport route from cytoplasm to chloroplast, Euglena was pulse labeled with 35S-sulfate and the organelles were separated on sucrose gradients. After a pulse, pSSU was found in the endoplasmic reticulum (ER) and Golgi apparatus. During a chase, ER-and Golgi-localized pSSU decreased concomitant with the appearance of SSU in chloroplasts. SSU was not found in pSSU-containing ER and Golgi fractions. Na2CO3 did not remove pSSU from ER or Golgi membranes, indicating that it was an integral membrane protein. pSSU was inserted in vitro into canine microsomes, and Na2CO3 did not remove pSSU from the microsomal membrane. The in vivo and in vitro experiments show that Euglena pSSU is inserted into the ER membrane and transported as an integral membrane protein to the Golgi apparatus before chloroplast import and polyprotein processing.

Entities: Chemical Species

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Year: 1996 PMID： 8597659 PMCID： PMC161080 DOI： 10.1105/tpc.8.1.43

Source DB: PubMed Journal: Plant Cell ISSN： 1040-4651 Impact factor: 11.277

16 in total

1. Stage-dependent localization of LHCP II apoprotein in the Golgi of synchronized cells of Euglena gracilis by immunogold electron microscopy.

Authors: T Osafune; J A Schiff; E Hase
Journal: Exp Cell Res Date: 1991-04 Impact factor: 3.905

2. Protein targeting across the three membranes of the Euglena chloroplast envelope.

Authors: L S Shashidhara; S H Lim; J B Shackleton; C Robinson; A G Smith
Journal: J Biol Chem Date: 1992-06-25 Impact factor: 5.157

3. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.

Authors: H Schägger; G von Jagow
Journal: Anal Biochem Date: 1987-11-01 Impact factor: 3.365

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

5. The presequence of Euglena LHCPII, a cytoplasmically synthesized chloroplast protein, contains a functional endoplasmic reticulum-targeting domain.

Authors: R Kishore; U S Muchhal; S D Schwartzbach
Journal: Proc Natl Acad Sci U S A Date: 1993-12-15 Impact factor: 11.205

6. Differences between lumen targeting domains of chloroplast transit peptides determine pathway specificity for thylakoid transport.

Authors: R Henry; A Kapazoglou; M McCaffery; K Cline
Journal: J Biol Chem Date: 1994-04-08 Impact factor: 5.157

7. Biogenesis of the yeast vacuole (lysosome). The precursor forms of the soluble hydrolase carboxypeptidase yscS are associated with the vacuolar membrane.

Authors: D O Spormann; J Heim; D H Wolf
Journal: J Biol Chem Date: 1992-04-25 Impact factor: 5.157

8. Expression and subcellular location of the tetrapyrrole synthesis enzyme porphobilinogen deaminase in light-grown Euglena gracilis and three nonchlorophyllous cell lines.

Authors: L S Shashidhara; A G Smith
Journal: Proc Natl Acad Sci U S A Date: 1991-01-01 Impact factor: 11.205

A soluble protein is imported into Euglena chloroplasts as a membrane-bound precursor.

1. Stage-dependent localization of LHCP II apoprotein in the Golgi of synchronized cells of Euglena gracilis by immunogold electron microscopy.

2. Protein targeting across the three membranes of the Euglena chloroplast envelope.

3. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

5. The presequence of Euglena LHCPII, a cytoplasmically synthesized chloroplast protein, contains a functional endoplasmic reticulum-targeting domain.

6. Differences between lumen targeting domains of chloroplast transit peptides determine pathway specificity for thylakoid transport.

7. Biogenesis of the yeast vacuole (lysosome). The precursor forms of the soluble hydrolase carboxypeptidase yscS are associated with the vacuolar membrane.

8. Expression and subcellular location of the tetrapyrrole synthesis enzyme porphobilinogen deaminase in light-grown Euglena gracilis and three nonchlorophyllous cell lines.

9. Photocontrol and processing of LHCP II apoprotein in Euglena: possible role of Golgi and other cytoplasmic sites.

10. Nucleotide sequence of two cDNAs encoding fucoxanthin chlorophyll a/c proteins in the diatom Odontella sinensis.

Review 1. Protein import into cyanelles and complex chloroplasts.

2. Phosphoglycerate kinase: structural aspects and functions, with special emphasis on the enzyme from Kinetoplastea.

3. Analysis of Euglena gracilis plastid-targeted proteins reveals different classes of transit sequences.

4. Tracing the evolution of the light-harvesting antennae in chlorophyll a/b-containing organisms.

5. The Lipid Composition of Euglena gracilis Middle Plastid Membrane Resembles That of Primary Plastid Envelopes.