Literature DB >> 8595858

Development of a genus specific primer set for detection of Leishmania parasites by polymerase chain reaction.

R Bhattacharyya1, K Das, S Sen, S Roy, H K Majumder.   

Abstract

We have compared the sequences of a major class of kinetoplast DNA (kDNA) minicircle (pLURkE3) of Leishmania strain UR6 with other minicircle sequences from different Leishmania species. Alignment of these sequences allowed the selection of a pair of oligonucleotides suitable as primers in polymerase chain reaction (PCR) which is specific for Leishmania parasites. PCR with this genus-specific primer set is capable of detecting 1 femtogram of kDNA. These primers have been tested with kDNAs from both old world and new world Leishmania species. The results indicate that the primers may be suitable for detection of any kind of leishmaniasis.

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Year:  1996        PMID： 8595858     DOI： 10.1111/j.1574-6968.1996.tb07989.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  6 in total

1.  Molecular identification of an old clinical isolate of Indian Kala-azar.

Authors:  Madhumita Manna; Supriya Khanra
Journal:  J Parasit Dis       Date:  2016-06-04

2.  Development of a species-specific PCR assay for detection of Leishmania donovani in clinical samples from patients with kala-azar and post-kala-azar dermal leishmaniasis.

Authors:  P Salotra; G Sreenivas; G P Pogue; N Lee; H L Nakhasi; V Ramesh; N S Negi
Journal:  J Clin Microbiol       Date:  2001-03       Impact factor: 5.948

3.  Identification and differentiation of Leishmania species in clinical samples by PCR amplification of the miniexon sequence and subsequent restriction fragment length polymorphism analysis.

Authors:  Jutta Marfurt; Abed Nasereddin; Igor Niederwieser; Charles L Jaffe; Hans-Peter Beck; Ingrid Felger
Journal:  J Clin Microbiol       Date:  2003-07       Impact factor: 5.948

4.  Lutzomyia sand fly diversity and rates of infection by Wolbachia and an exotic Leishmania species on Barro Colorado Island, Panama.

Authors:  Jorge Azpurua; Dianne De La Cruz; Anayansi Valderama; Donald Windsor
Journal:  PLoS Negl Trop Dis       Date:  2010-03-09

5.  Single-step multiplex PCR assay for characterization of New World Leishmania complexes.

Authors:  E Harris; G Kropp; A Belli; B Rodriguez; N Agabian
Journal:  J Clin Microbiol       Date:  1998-07       Impact factor: 5.948

6.  Molecular identification of leishmania species using samples obtained from negative stained smears.

Authors:  Ma Mohaghegh; A Fata; Gh Salehi; F Berenji; M Mousavi Bazzaz; H Rafatpanah; M Parian; A Movahedi
Journal:  Iran J Parasitol       Date:  2013-04       Impact factor: 1.012
  6 in total

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