Literature DB >> 8594985

A colorimetric method for the determination of lipoxygenase activity suitable for use in a high throughput assay format.

N B Waslidge1, D J Hayes.   

Abstract

A rapid and sensitive method for measuring the activity of lipoxygenase is described. The assay is based on the principle that under acidic conditions a lipid hydroperoxide can oxidize Fe2+ to Fe3+ which then oxidizes xylenol orange to form a product that absorbs strongly in the visible region (see Z-Y. Jiang, A. C. S. Woollard, and S. P. Wolff, Lipids 26, 853-856, 1991). This methodology was modified to measure lipoxygenase activity and the system was optimized using platelet 12-lipoxygenase. This assay is suitable for use in a 96-well microtiter plate and may be utilized as a high throughput screen for the identification of novel lipoxygenase inhibitors.

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Year:  1995        PMID： 8594985     DOI： 10.1006/abio.1995.0063

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  11 in total

1.  Synthesis and evaluation of 5-lipoxygenase translocation inhibitors from acylnitroso hetero-Diels-Alder cycloadducts.

Authors:  Joshua K Bolger; Wen Tian; William R Wolter; Wonhwa Cho; Mark A Suckow; Marvin J Miller
Journal:  Org Biomol Chem       Date:  2011-03-02       Impact factor: 3.876

2.  A high-throughput mass spectrometric assay for discovery of human lipoxygenase inhibitors and allosteric effectors.

Authors:  J Brian Jameson; Victor Kenyon; Theodore R Holman
Journal:  Anal Biochem       Date:  2015-02-21       Impact factor: 3.365

3.  Modulation of arachidonic acid distribution by conjugated linoleic acid isomers and linoleic acid in MCF-7 and SW480 cancer cells.

Authors:  A Miller; C Stanton; R Devery
Journal:  Lipids       Date:  2001-10       Impact factor: 1.880

4.  Lipoxygenases and poly(ADP-ribose) polymerase in amyloid beta cytotoxicity.

Authors:  Joanna B Strosznajder; Magdalena Cieslik; Magdalena Cakala; Henryk Jesko; Anne Eckert; Robert P Strosznajder
Journal:  Neurochem Res       Date:  2011-02-02       Impact factor: 3.996

5.  Discovery of platelet-type 12-human lipoxygenase selective inhibitors by high-throughput screening of structurally diverse libraries.

Authors:  Joshua D Deschamps; Jeffrey T Gautschi; Stephanie Whitman; Tyler A Johnson; Nadine C Gassner; Phillip Crews; Theodore R Holman
Journal:  Bioorg Med Chem       Date:  2007-08-22       Impact factor: 3.641

6.  Pseudoperoxidase investigations of hydroperoxides and inhibitors with human lipoxygenases.

Authors:  Eric K Hoobler; Charles Holz; Theodore R Holman
Journal:  Bioorg Med Chem       Date:  2013-04-18       Impact factor: 3.641

7.  Structure-activity relationship studies of flavonoids as potent inhibitors of human platelet 12-hLO, reticulocyte 15-hLO-1, and prostate epithelial 15-hLO-2.

Authors:  Yesseny Vasquez-Martinez; Rachana V Ohri; Victor Kenyon; Theodore R Holman; Silvia Sepúlveda-Boza
Journal:  Bioorg Med Chem       Date:  2007-08-22       Impact factor: 3.641

8.  Targeting ferroptosis: A novel therapeutic strategy for the treatment of mitochondrial disease-related epilepsy.

Authors:  Amanda H Kahn-Kirby; Akiko Amagata; Celine I Maeder; Janet J Mei; Steve Sideris; Yuko Kosaka; Andrew Hinman; Stephanie A Malone; Joel J Bruegger; Leslie Wang; Virna Kim; William D Shrader; Kevin G Hoff; Joey C Latham; Euan A Ashley; Matthew T Wheeler; Enrico Bertini; Rosalba Carrozzo; Diego Martinelli; Carlo Dionisi-Vici; Kimberly A Chapman; Gregory M Enns; William Gahl; Lynne Wolfe; Russell P Saneto; Simon C Johnson; Jeffrey K Trimmer; Matthew B Klein; Charles R Holst
Journal:  PLoS One       Date:  2019-03-28       Impact factor: 3.240

9.  Role of the lipoxygenase pathway in RSV-induced alternatively activated macrophages leading to resolution of lung pathology.

Authors:  K A Shirey; W Lai; L M Pletneva; C L Karp; S Divanovic; J C G Blanco; S N Vogel
Journal:  Mucosal Immunol       Date:  2013-09-25       Impact factor: 7.313

10.  A Xylenol Orange-Based Screening Assay for the Substrate Specificity of Flavin-Dependent para-Phenol Oxidases.

Authors:  Tom A Ewing; Aster van Noord; Caroline E Paul; Willem J H van Berkel
Journal:  Molecules       Date:  2018-01-14       Impact factor: 4.411
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