Isolation and characterization of a protein corresponding to mKlk-11 clone from male mouse submandibular gland.

A protein corresponding to the predicted genomic sequence of clone mKlk-11 has been characterized from mouse submandibular gland. This protein was purified by reverse-phase high-performance liquid chromatography from the submandibular glands of normal and hypertensive mice. The protein was not detected in the submandibular gland of mice selected for low blood pressure. It consists of three fragments starting at the residues 1, 98, and 141 of the predicted sequence of clone mKlk-11. The cleavage of beta-lactoglobulin (between residues 20 and 21, Tyr-Ser, and 40 and 41, Arg-Val) and a synthetic renin substrate tetradecapeptide (residues 4 and 5, Tyr-Ile) by the protein corresponding to clone mKlk-11 showed both tryptic- and chymotryptic-type cleavages. The possibility of this protein's involvement in the regulation of local blood flow is raised.