Production and characterisation of anti-cardiac troponin-I monoclonal antibodies.

Cardiac troponin-I (cTn-I) was isolated from bovine left ventricular tissue and used as immunogen. Sixteen murine hybridoma lines were produced with two of them, 1D12 and 5F4, showing a high specificity for cTn-I; both of these monoclonal antibodies (McAbs) were isotyped as IgG1 with kappa-light chains. The specificity of the McAbs for cTn-I was confirmed by ELISA, western blotting and by the ability of the antibodies to block actomyosin ATPase inhibition by cTn-I. The McAbs may be useful for human in vivo imaging of myocardial infarcts and other pathological conditions related to cardiac myocyte damage.