| Literature DB >> 8589843 |
Abstract
The entire insect mitochondrial genome has been amplified in just two PCR reactions. 'Universal' insect primers and techniques to optimize amplification of long PCR products were used to obtain segments of the mitochondrial genomes of Helicoverpa zea and Heliothis virescens (Lepidoptera) ranging in size from 3.7 kb to 14 kb or about 25% to over 85% of a typical 16 kb mtDNA. Overlapping pairs of fragments contain the complete mtDNA sequence.Entities:
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Year: 1995 PMID： 8589843 DOI： 10.1111/j.1365-2583.1995.tb00022.x
Source DB: PubMed Journal: Insect Mol Biol ISSN： 0962-1075 Impact factor: 3.585