Literature DB >> 858912

An electron stain for elastic fibers using orcein.

H Nakamura, C Kanai, V Mizuhira.   

Abstract

Orcein was found to be useful as an electron-opaque stain for elastic fibers in epoxy-sections. Ultra-thin sections of aorta were treated with elastica stain containing 0.1-0.3% orcein and counterstained in uranyl acetate and lead citrate. Elastic fibers were densely and specifically demonstrated in the stroma and near smooth muscle cells. The result of orcein staining has a comparable appearance under both light and electron microscopes.

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Year:  1977        PMID： 858912     DOI： 10.1177/25.4.858912

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN： 0022-1554            Impact factor:   2.479


  10 in total

1.  Orcein, collastin and pseudo-elastica: a re-investigation of Unna's concepts.

Authors:  H Puchtler; S N Meloan
Journal:  Histochemistry       Date:  1979-11

2.  Distribution and organization of the elastic system fibres in healthy human gingiva. Ultrastructural and immunohistochemical study.

Authors:  C Chavrier; D J Hartmann; M L Couble; D Herbage
Journal:  Histochemistry       Date:  1988

3.  The lamina propria of the bovine seminiferous tubule.

Authors:  K H Wrobel; R Mademann; F Sinowatz
Journal:  Cell Tissue Res       Date:  1979-11       Impact factor: 5.249

4.  Ultrastructural changes of venous autologous bypass grafts in rabbits: correlation of patency and development.

Authors:  M Bergmann; N Walther
Journal:  Basic Res Cardiol       Date:  1982 Nov-Dec       Impact factor: 17.165

5.  Ultrastructural visualization of elastic fibres with a tannate--metal salt method.

Authors:  M Kageyama; M Takagi; R T Parmley; M Toda; H Hirayama; Y Toda
Journal:  Histochem J       Date:  1985-01

6.  Postnatal overnutrition in mice leads to impaired pulmonary mechanics in response to salbutamol.

Authors:  Vanessa P Teixeira; Daniela A B Cervilha; Layla D M Cabral; Luiz M Oliveira; Erika K Incerpi; Rômulo D Novaes; Marisa Ionta; Roseli Soncini
Journal:  J Physiol Sci       Date:  2015-10-23       Impact factor: 2.781

7.  Elastic fibers associated with collagenous fibrils surrounded by reticular cells in lymph nodes of the rat as revealed by electron microscopy after orcein staining.

Authors:  K Miyata; K Takaya
Journal:  Cell Tissue Res       Date:  1981       Impact factor: 5.249

8.  Annulo-aortic ectasia. Light and electron microscopic changes in aortic media.

Authors:  T Savunen; H J Aho
Journal:  Virchows Arch A Pathol Anat Histopathol       Date:  1985

9.  Elastic microfibril distribution in the cornea: Differences between normal and keratoconic stroma.

Authors:  Tomas L White; Philip N Lewis; Robert D Young; Koji Kitazawa; Tsutomu Inatomi; Shigeru Kinoshita; Keith M Meek
Journal:  Exp Eye Res       Date:  2017-03-14       Impact factor: 3.467

10.  Three-dimensional arrangement of elastic fibers in the human corneal stroma.

Authors:  Philip N Lewis; Tomas L White; Robert D Young; James S Bell; C Peter Winlove; Keith M Meek
Journal:  Exp Eye Res       Date:  2015-12-17       Impact factor: 3.467
  10 in total

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