Literature DB >> 8587007

DNA extraction and amplification from Giemsa-stained blood smears.

M Yokota1, N Tatsumi, I Tsuda, I Yano.   

Abstract

DNA extraction was attempted from Giemsa-stained blood smears on glass slides that had been stored for several years. High molecular weight DNA bands were clearly visible after electrophoresis when DNA was extracted from specimens stored up to 2 years. Specimens more than 4 years old demonstrated a smeared pattern, suggesting degeneration of the DNA, but they could be rescued by PCR amplification using primers of HLA-DQA1 genes. The recovery could be pursued even in 11-year-old specimens.

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Year:  1995        PMID： 8587007     DOI： 10.1002/jcla.1860090609

Source DB:  PubMed          Journal:  J Clin Lab Anal        ISSN： 0887-8013            Impact factor:   2.352


  9 in total

1.  Effects of heparin on polymerase chain reaction for blood white cells.

Authors:  M Yokota; N Tatsumi; O Nathalang; T Yamada; I Tsuda
Journal:  J Clin Lab Anal       Date:  1999       Impact factor: 2.352

2.  Analytical Performance of a Loop-Mediated Isothermal Amplification Assay for Leishmania DNA Detection in Sandflies and Direct Smears of Patients with Cutaneous Leishmaniasis.

Authors:  Cielo M León; Marina Muñoz; Juan H Tabares; Carolina Hernandez; Carolina Florez; Martha S Ayala; Juan David Ramírez
Journal:  Am J Trop Med Hyg       Date:  2018-03-08       Impact factor: 2.345

3.  The PCR-based detection and identification of the parasites causing human cutaneous leishmaniasis in the Iranian city of Ahvaz.

Authors:  M Ghasemian; S Maraghi; A R Samarbafzadeh; A Jelowdar; M Kalantari
Journal:  Ann Trop Med Parasitol       Date:  2011-04

4.  Postmortem molecular diagnosis of sickle beta thalassaemia.

Authors:  F Kutlar; D Mirmow; M Glendenning; L Holley; A Kutlar
Journal:  J Clin Pathol       Date:  2005-05       Impact factor: 3.411

5.  Cutaneous and post kala-azar dermal leishmaniasis caused by Leishmania infantum in endemic areas of visceral leishmaniasis, northwestern Iran 2002-2011: a case series.

Authors:  Alireza Badirzadeh; Mehdi Mohebali; Mehrdad Ghasemian; Hassan Amini; Zabiholah Zarei; Behnaz Akhoundi; Homa Hajjaran; Dariush Emdadi; Soheila Molaei; Ahmad Kusha; Saber Alizadeh
Journal:  Pathog Glob Health       Date:  2013-06       Impact factor: 2.894

6.  Comparison of Fluorometric and UV Spectrophotometric Findings for DNA Isolated From Formalin-Fixed Paraffin-Embedded Blocks, Fine Needle Aspiration Cytology Smears, and Blood.

Authors:  Pranoy Paul; Swati Rajput; Prashant Joshi; Manisha Naithani; Nilotpal Chowdhury; Shalinee Rao; Manju O Pai
Journal:  Cureus       Date:  2021-11-15

7.  Plasmodium knowlesi from archival blood films: further evidence that human infections are widely distributed and not newly emergent in Malaysian Borneo.

Authors:  Kim-Sung Lee; Janet Cox-Singh; George Brooke; Asmad Matusop; Balbir Singh
Journal:  Int J Parasitol       Date:  2009-04-07       Impact factor: 3.981

8.  Evaluation of microRNA expression in patient bone marrow aspirate slides.

Authors:  Leah Morenos; Richard Saffery; Francoise Mechinaud; David Ashley; Ngaire Elwood; Jeffrey M Craig; Nicholas C Wong
Journal:  PLoS One       Date:  2012-08-13       Impact factor: 3.240

9.  High proportion of knowlesi malaria in recent malaria cases in Malaysia.

Authors:  Ruhani Yusof; Yee Ling Lau; Rohela Mahmud; Mun Yik Fong; Jenarun Jelip; Hie Ung Ngian; Sahlawati Mustakim; Hani Mat Hussin; Noradilah Marzuki; Marlindawati Mohd Ali
Journal:  Malar J       Date:  2014-05-03       Impact factor: 2.979
  9 in total

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